Caffeic acid phenethyl ester protects 661W cells from H 2 O 2 - mediated cell death and enhances electroretinography response in dim-reared albino rats

Hui Chen, Julie Thu A. Tran, Robert E. Anderson, Nawajes Mandal

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H 2 O 2 -mediated cell death and in albino rats (in vivo) against various light conditions. Methods: The 661W cells were pretreated with CAPE and then stressed with H 2 O 2 . Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02%) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding. Results: Pretreatment of 661W cells with CAPE reduced H 2 O 2 -mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light. Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.

Original languageEnglish (US)
Pages (from-to)1325-1338
Number of pages14
JournalMolecular Vision
Volume18
StatePublished - May 30 2012
Externally publishedYes

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Electroretinography
Cell Death
Retina
Heme Oxygenase-1
Light
Arachidonate 12-Lipoxygenase
Propolis
Lipids
caffeic acid phenethyl ester
Messenger RNA
Proteins
Omega-3 Fatty Acids
Intercellular Adhesion Molecule-1
L-Lactate Dehydrogenase
Sprague Dawley Rats
Histology
Diet
Gene Expression
Antigens

All Science Journal Classification (ASJC) codes

  • Ophthalmology

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Caffeic acid phenethyl ester protects 661W cells from H 2 O 2 - mediated cell death and enhances electroretinography response in dim-reared albino rats . / Chen, Hui; Tran, Julie Thu A.; Anderson, Robert E.; Mandal, Nawajes.

In: Molecular Vision, Vol. 18, 30.05.2012, p. 1325-1338.

Research output: Contribution to journalArticle

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abstract = "Purpose: Caffeic acid phenethyl ester (CAPE), an active component of honeybee propolis, has a wide range of beneficial properties. The purpose of this study was to test the protective role of CAPE in 661W cells (in vitro) against H 2 O 2 -mediated cell death and in albino rats (in vivo) against various light conditions. Methods: The 661W cells were pretreated with CAPE and then stressed with H 2 O 2 . Cell death was measured with lactate dehydrogenase (LDH) release assay, and mRNA and proteins were analyzed. Sprague Dawley rats were raised on either a control or CAPE (0.02{\%}) diet and exposed to various light conditions for short or long periods. Retinal histology, mRNA, protein, lipid composition, and retinal function by electroretinography (ERG) were measured at the end of feeding. Results: Pretreatment of 661W cells with CAPE reduced H 2 O 2 -mediated cell death in a dose-dependent manner and induced expression of heme oxygenase-1 (Ho1). Albino rats fed with CAPE had greater expression of Ho1 and intercellular adhesion molecule 1 (Icam1), less expression of FOS-like antigen (Fosl) and lipoxygenase 12 (Lox12) genes in the retina, less translocation of nuclear factor kappaB protein to the nucleus, and a lower molar ratio of n-3 polyunsaturated fatty acids. Further, the ERGs of the retinas of CAPE-fed rats were significantly higher than those of the control-fed rats when raised in dim light. Conclusions: CAPE can activate the antioxidative gene expression pathway in retinal cells in vitro and in vivo. Feeding CAPE to albino rats can enhance ERG responses and change the lipid profile in the rats' retinas.",
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