Calcifying epithelial odontogenic (Pindborg) tumor-associated amyloid consists of a novel human protein

Alan Solomon, Charles L. Murphy, Kristal Weaver, Deborah T. Weiss, Rudi Hrncic, Manfred Eulitz, Robert L. Donnell, Knut Sletten, Gunilla Westermark, Per Westermark

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Calcifying epithelial odontogenic tumors (CEOTs), also known as Pindborg tumors, are characterized by the presence of squamous-cell proliferation, calcification, and, notably, amyloid deposits. On the basis of immunohistochemical analyses, the amyloidogenic component had heretofore been deemed to consist of cytokeratin-related or other molecules; however, its chemical composition had never been elucidated. We have used our microanalytic techniques to characterize the protein nature of CEOT-associated amyloid isolated from specimens obtained from 3 patients. As evidenced by the results of amino-acid sequencing and mass spectrometry, the fibrils were found to be composed of a polypeptide of approximately 46 mer. This component was identical in sequence to the N-terminal portion of a hypothetical 153-residue protein encoded by the FLJ20513 gene cloned from the human KATO III cell line. That the amyloid protein was derived from this larger molecule was demonstrated by reverse transcription-polymerase chain reaction amplification of tumor-cell RNA where a full-length FLJ20513 transcript was found. Furthermore, immunohistochemical analyses revealed that the amyloid within the CEOTs immunostained with antibodies prepared against a synthetic FLJ20513-related dodecapeptide. Our studies provide unequivocal evidence that CEOT-associated amyloid consists of a unique and previously undescribed protein that we provisionally designate APin.

Original languageEnglish (US)
Pages (from-to)348-355
Number of pages8
JournalJournal of Laboratory and Clinical Medicine
Volume142
Issue number5
DOIs
StatePublished - Jan 1 2003

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Amyloid
Tumors
Proteins
Amyloidogenic Proteins
Protein Sequence Analysis
Amyloid Plaques
Keratins
Molecules
Polymerase chain reaction
Cell proliferation
Reverse Transcription
Transcription
Mass Spectrometry
Epithelial Cells
Mass spectrometry
Amplification
Cell Proliferation
Calcifying Epithelial Odontogenic Tumor
RNA
Deposits

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Calcifying epithelial odontogenic (Pindborg) tumor-associated amyloid consists of a novel human protein. / Solomon, Alan; Murphy, Charles L.; Weaver, Kristal; Weiss, Deborah T.; Hrncic, Rudi; Eulitz, Manfred; Donnell, Robert L.; Sletten, Knut; Westermark, Gunilla; Westermark, Per.

In: Journal of Laboratory and Clinical Medicine, Vol. 142, No. 5, 01.01.2003, p. 348-355.

Research output: Contribution to journalArticle

Solomon, A, Murphy, CL, Weaver, K, Weiss, DT, Hrncic, R, Eulitz, M, Donnell, RL, Sletten, K, Westermark, G & Westermark, P 2003, 'Calcifying epithelial odontogenic (Pindborg) tumor-associated amyloid consists of a novel human protein', Journal of Laboratory and Clinical Medicine, vol. 142, no. 5, pp. 348-355. https://doi.org/10.1016/S0022-2143(03)00149-5
Solomon, Alan ; Murphy, Charles L. ; Weaver, Kristal ; Weiss, Deborah T. ; Hrncic, Rudi ; Eulitz, Manfred ; Donnell, Robert L. ; Sletten, Knut ; Westermark, Gunilla ; Westermark, Per. / Calcifying epithelial odontogenic (Pindborg) tumor-associated amyloid consists of a novel human protein. In: Journal of Laboratory and Clinical Medicine. 2003 ; Vol. 142, No. 5. pp. 348-355.
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