Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans

Jaya Bandyopadhyay, Jiyeon Lee, Jungsoo Lee, Jin Il Lee, Jae Ran Yu, Chang Hoon Jee, Jeong Hoon Cho, Sunki Jung, Myon Hee Lee, Sonia Zannoni, Andrew Singson, Do Han Kim, Hyeon Sook Koo, Joohong Ahnn

Research output: Contribution to journalArticle

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Abstract

Calcineurin is a Ca2+-calmodulin-dependent serine/threonine protein phosphatase that has been implicated in various signaling pathways. Here we report the identification and characterization of calcineurin genes in Caenorhabditis elegans (cna-1 and cnb-1), which share high homology with Drosophila and mammalian calcineurin genes. C. elegans calcineurin binds calcium and functions as a heterodimeric protein phosphatase establishing its biochemical conservation in the nematode. Calcineurin is expressed in hypodermal seam cells, body-wall muscle, vulva muscle, neuronal cells, and in sperm and the spermatheca. cnb-1 mutants showed pleiotropic defects including lethargic movement and delayed egg-laying. Interestingly, these characteristic defects resembled phenotypes observed in gain-of-function mutants of unc-43/Ca2+-calmodulin-dependent protein kinase II (CaMKII) and goa-1/Go-protein α-subunit. Double mutants of cnb-1 and unc-43(gf) displayed an apparent synergistic severity of movement and egg-laying defects, suggesting that caldneurin may have an antagonistic role in CaMKII-regulated phosphorylation signaling pathways in C. elegans.

Original languageEnglish (US)
Pages (from-to)3281-3293
Number of pages13
JournalMolecular Biology of the Cell
Volume13
Issue number9
DOIs
StatePublished - Sep 1 2002

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Calcineurin
Phosphoprotein Phosphatases
Caenorhabditis elegans
Calmodulin
Ovum
Fertility
Calcium
Growth
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Vulva
Protein Subunits
Cell Wall
Muscle Cells
Genes
Drosophila
Spermatozoa
Phosphorylation
Phenotype
Muscles

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans. / Bandyopadhyay, Jaya; Lee, Jiyeon; Lee, Jungsoo; Lee, Jin Il; Yu, Jae Ran; Jee, Chang Hoon; Cho, Jeong Hoon; Jung, Sunki; Lee, Myon Hee; Zannoni, Sonia; Singson, Andrew; Kim, Do Han; Koo, Hyeon Sook; Ahnn, Joohong.

In: Molecular Biology of the Cell, Vol. 13, No. 9, 01.09.2002, p. 3281-3293.

Research output: Contribution to journalArticle

Bandyopadhyay, J, Lee, J, Lee, J, Lee, JI, Yu, JR, Jee, CH, Cho, JH, Jung, S, Lee, MH, Zannoni, S, Singson, A, Kim, DH, Koo, HS & Ahnn, J 2002, 'Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans', Molecular Biology of the Cell, vol. 13, no. 9, pp. 3281-3293. https://doi.org/10.1091/mbc.E02-01-0005
Bandyopadhyay, Jaya ; Lee, Jiyeon ; Lee, Jungsoo ; Lee, Jin Il ; Yu, Jae Ran ; Jee, Chang Hoon ; Cho, Jeong Hoon ; Jung, Sunki ; Lee, Myon Hee ; Zannoni, Sonia ; Singson, Andrew ; Kim, Do Han ; Koo, Hyeon Sook ; Ahnn, Joohong. / Calcineurin, a calcium/calmodulin-dependent protein phosphatase, is involved in movement, fertility, egg laying, and growth in Caenorhabditis elegans. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 9. pp. 3281-3293.
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