Calcineurin regulates enteric muscle contraction through EXP-1, excitatory GABA-gated channel, in C. elegans

Jungsoo Lee, Hyun Ok Song, Chang Hoon Jee, Liviu Vanoaica, Joohong Ahnn

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The enteric muscle contraction (EMC) is the last step of the defecation behavior which occurs every 50 s in Caenorhabditis elegans. This EMC is regulated by intestinal and anal depressor muscles, which are innervated by GABA motor neurons. Our data show that calcineurin (tax-6) is expressed in intestinal muscle and anal depressor muscle, and the gain-of-function mutant of calcineurin, tax-6(jh107), shows defects in enteric muscle contractions. In addition, the intracellular region of EXP-1, an excitatory GABA receptor, specifically binds to calcineurin A. This interaction between TAX-6 and EXP-1 appears to be independent of both calcium and CNB, which is the calcium-binding regulatory subunit. Genetic evidence of epistasis between cnb-1(jh103) and exp-1(sa6) suggests that calcineurin functions as a negative regulator of excitatory GABA receptor in GABA signaling in C. elegans.

Original languageEnglish (US)
Pages (from-to)313-318
Number of pages6
JournalJournal of Molecular Biology
Volume352
Issue number2
DOIs
StatePublished - Sep 16 2005

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Calcineurin
Muscle Contraction
gamma-Aminobutyric Acid
GABA Receptors
Caenorhabditis elegans
Muscles
Genetic Epistasis
Calcium
GABAergic Neurons
Defecation
Motor Neurons

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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Calcineurin regulates enteric muscle contraction through EXP-1, excitatory GABA-gated channel, in C. elegans. / Lee, Jungsoo; Song, Hyun Ok; Jee, Chang Hoon; Vanoaica, Liviu; Ahnn, Joohong.

In: Journal of Molecular Biology, Vol. 352, No. 2, 16.09.2005, p. 313-318.

Research output: Contribution to journalArticle

Lee, Jungsoo ; Song, Hyun Ok ; Jee, Chang Hoon ; Vanoaica, Liviu ; Ahnn, Joohong. / Calcineurin regulates enteric muscle contraction through EXP-1, excitatory GABA-gated channel, in C. elegans. In: Journal of Molecular Biology. 2005 ; Vol. 352, No. 2. pp. 313-318.
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