Candida albicans in oral biofilms could prevent caries

Hubertine Willems, Kevin Kos, Mary Ann Jabra-Rizk, Bastiaan P. Krom

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Streptococcus mutans is a Gram-positive bacterium involved in development to caries, the most common infectious disease of our time. Streptococcus mutans interacts with other microbes, like the fungus Candida albicans and both are commonly isolated from patients with caries. Since the role of C. albicans in caries remains unknown, our aim was to unravel this using an in vitro dual-species cariogenic oral biofilm model. Biofilms were grown for 24-72 h on glass cover slips or hydroxyapatite (HA) disks to mimic the surface of teeth. Medium pH, lactic acid production capacity and calcium release from HA disks were determined. All 24-h biofilms had external pH values below the critical pH of 5.5 where enamel dissolves. In contrast, 72-h dual-species biofilms had significantly higher pH (above the critical pH) and consequently decreased calcium release compared to single-species S. mutans biofilms. Counter intuitively, lactic acid production and growth of S. mutans were increased in 72-h dual-species biofilms. Candida albicans modulates the pH in dual-species biofilms to values above the critical pH where enamel dissolves. Our results suggest that C. albicans is not by definition a cariogenic microorganism; it could prevent caries by actively increasing pH preventing mineral loss.

Original languageEnglish (US)
JournalPathogens and Disease
Volume74
Issue number5
DOIs
StatePublished - Jul 1 2016

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Biofilms
Candida albicans
Streptococcus mutans
Durapatite
Dental Enamel
Lactic Acid
Gram-Positive Bacteria
Glass
Minerals
Communicable Diseases
Tooth
Fungi
Calcium
Growth

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Candida albicans in oral biofilms could prevent caries. / Willems, Hubertine; Kos, Kevin; Jabra-Rizk, Mary Ann; Krom, Bastiaan P.

In: Pathogens and Disease, Vol. 74, No. 5, 01.07.2016.

Research output: Contribution to journalArticle

Willems, Hubertine ; Kos, Kevin ; Jabra-Rizk, Mary Ann ; Krom, Bastiaan P. / Candida albicans in oral biofilms could prevent caries. In: Pathogens and Disease. 2016 ; Vol. 74, No. 5.
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