Carbachol stimulates [35S]guanylyl 5'-(γ-thio)-triphosphate binding in rapid eye movement sleep-related brainstem nuclei of rat

M. Luisa Capece, Helen Baghdoyan, Ralph Lydic

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Abstract

Carbachol enhances rapid eye movement (REM) sleep when microinjected into the pontine reticular formation of the cat and rat. Carbachol elicits this REM sleep-like state via activation of postsynaptic muscarinic cholinergic receptors (mAChRs). The present study used in vitro autoradiography of carbacholstimulated [35S]guanylyl-5'-O-(γ-thio)- triphosphate ([35S]GTPγS) binding to test the hypothesis that carbachol activates mAChRs to induce stimulation of G-proteins in brainstem nuclei contributing to REM sleep generation. The results demonstrate a heterogeneous increase in carbachol-stimulated G-protein activation across rat brainstem. Binding of [35S]GTPγS in the presence of carbachol, compared with basal binding, was significantly increased in the laterodorsal tegmental nucleus (75.7%), caudal pontine reticular nucleus (68.9%), oral pontine reticular nucleus (64.5%), pedunculopontine tegmental nucleus (55.7%), and dorsal raphe nucleus (54.0%) but not in the nucleus locus coeruleus. The activation of G- proteins by carbachol was concentration-dependent and antagonized by atropine, demonstrating that G-proteins were activated via mAChR stimulation. The results provide the first direct measures of mAChR-activated G-proteins in brainstem nuclei known to contribute to REM sleep generation.

Original languageEnglish (US)
Pages (from-to)3779-3785
Number of pages7
JournalJournal of Neuroscience
Volume18
Issue number10
StatePublished - May 15 1998
Externally publishedYes

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REM Sleep
Carbachol
Brain Stem
Sleep
GTP-Binding Proteins
Cholinergic Receptors
Muscarinic Receptors
Pedunculopontine Tegmental Nucleus
Locus Coeruleus
Autoradiography
Atropine
triphosphoric acid
Cats

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

@article{019adae9bc3647ce9c49cbb96ba50072,
title = "Carbachol stimulates [35S]guanylyl 5'-(γ-thio)-triphosphate binding in rapid eye movement sleep-related brainstem nuclei of rat",
abstract = "Carbachol enhances rapid eye movement (REM) sleep when microinjected into the pontine reticular formation of the cat and rat. Carbachol elicits this REM sleep-like state via activation of postsynaptic muscarinic cholinergic receptors (mAChRs). The present study used in vitro autoradiography of carbacholstimulated [35S]guanylyl-5'-O-(γ-thio)- triphosphate ([35S]GTPγS) binding to test the hypothesis that carbachol activates mAChRs to induce stimulation of G-proteins in brainstem nuclei contributing to REM sleep generation. The results demonstrate a heterogeneous increase in carbachol-stimulated G-protein activation across rat brainstem. Binding of [35S]GTPγS in the presence of carbachol, compared with basal binding, was significantly increased in the laterodorsal tegmental nucleus (75.7{\%}), caudal pontine reticular nucleus (68.9{\%}), oral pontine reticular nucleus (64.5{\%}), pedunculopontine tegmental nucleus (55.7{\%}), and dorsal raphe nucleus (54.0{\%}) but not in the nucleus locus coeruleus. The activation of G- proteins by carbachol was concentration-dependent and antagonized by atropine, demonstrating that G-proteins were activated via mAChR stimulation. The results provide the first direct measures of mAChR-activated G-proteins in brainstem nuclei known to contribute to REM sleep generation.",
author = "Capece, {M. Luisa} and Helen Baghdoyan and Ralph Lydic",
year = "1998",
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T1 - Carbachol stimulates [35S]guanylyl 5'-(γ-thio)-triphosphate binding in rapid eye movement sleep-related brainstem nuclei of rat

AU - Capece, M. Luisa

AU - Baghdoyan, Helen

AU - Lydic, Ralph

PY - 1998/5/15

Y1 - 1998/5/15

N2 - Carbachol enhances rapid eye movement (REM) sleep when microinjected into the pontine reticular formation of the cat and rat. Carbachol elicits this REM sleep-like state via activation of postsynaptic muscarinic cholinergic receptors (mAChRs). The present study used in vitro autoradiography of carbacholstimulated [35S]guanylyl-5'-O-(γ-thio)- triphosphate ([35S]GTPγS) binding to test the hypothesis that carbachol activates mAChRs to induce stimulation of G-proteins in brainstem nuclei contributing to REM sleep generation. The results demonstrate a heterogeneous increase in carbachol-stimulated G-protein activation across rat brainstem. Binding of [35S]GTPγS in the presence of carbachol, compared with basal binding, was significantly increased in the laterodorsal tegmental nucleus (75.7%), caudal pontine reticular nucleus (68.9%), oral pontine reticular nucleus (64.5%), pedunculopontine tegmental nucleus (55.7%), and dorsal raphe nucleus (54.0%) but not in the nucleus locus coeruleus. The activation of G- proteins by carbachol was concentration-dependent and antagonized by atropine, demonstrating that G-proteins were activated via mAChR stimulation. The results provide the first direct measures of mAChR-activated G-proteins in brainstem nuclei known to contribute to REM sleep generation.

AB - Carbachol enhances rapid eye movement (REM) sleep when microinjected into the pontine reticular formation of the cat and rat. Carbachol elicits this REM sleep-like state via activation of postsynaptic muscarinic cholinergic receptors (mAChRs). The present study used in vitro autoradiography of carbacholstimulated [35S]guanylyl-5'-O-(γ-thio)- triphosphate ([35S]GTPγS) binding to test the hypothesis that carbachol activates mAChRs to induce stimulation of G-proteins in brainstem nuclei contributing to REM sleep generation. The results demonstrate a heterogeneous increase in carbachol-stimulated G-protein activation across rat brainstem. Binding of [35S]GTPγS in the presence of carbachol, compared with basal binding, was significantly increased in the laterodorsal tegmental nucleus (75.7%), caudal pontine reticular nucleus (68.9%), oral pontine reticular nucleus (64.5%), pedunculopontine tegmental nucleus (55.7%), and dorsal raphe nucleus (54.0%) but not in the nucleus locus coeruleus. The activation of G- proteins by carbachol was concentration-dependent and antagonized by atropine, demonstrating that G-proteins were activated via mAChR stimulation. The results provide the first direct measures of mAChR-activated G-proteins in brainstem nuclei known to contribute to REM sleep generation.

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