Cardiac arrest-induced complete cerebral ischaemia in the rat

Dynamics of postischaemic in vivo calcium uptake and protein synthesis

G. Mies, K. Kawai, N. Saito, G. Nagashima, Thaddeus Nowak, C. A. Ruetzler, I. Klatzo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Dynamics of pathological changes following cardiac arrest induced cerebral ischaemia were related to the findings of double tracer autoradiography of 45Ca and 3H leucine uptake as respective indicators of ischaemic injury and metabolic disturbance. Abnormal calcium accumulation, determined by 45Ca uptake, was related to injured but still living neurons and to reactive glial elements. 45Ca autoradiography confirmed a high sensitivity to neuronal injury of the nucleus reticularis thalami (NRT), hippocampal CA1 pyramidal layer, inferior colliculus, ventral thalamic nucleus (VTN), caudate nucleus, and parietal cortex. 3H leucine incorporation revealed that an initially widespread inhibition of protein synthesis was followed by its considerable recovery. Observations concerning hippocampal CA1 sector and VTN suggested that a significant degree of protein synthesis, maintained at the late stage after postischaemic recovery, was related to survival and regeneration of neurons and not to the presence of glial elements.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalNeurological Research
Volume15
Issue number4
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Ventral Thalamic Nuclei
Induced Heart Arrest
Brain Ischemia
Autoradiography
Leucine
Neuroglia
Calcium
Hippocampal CA1 Region
Neurons
Inferior Colliculi
Parietal Lobe
Caudate Nucleus
Wounds and Injuries
Thalamus
Regeneration
Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology

Cite this

Cardiac arrest-induced complete cerebral ischaemia in the rat : Dynamics of postischaemic in vivo calcium uptake and protein synthesis. / Mies, G.; Kawai, K.; Saito, N.; Nagashima, G.; Nowak, Thaddeus; Ruetzler, C. A.; Klatzo, I.

In: Neurological Research, Vol. 15, No. 4, 01.01.1993, p. 253-263.

Research output: Contribution to journalArticle

Mies, G. ; Kawai, K. ; Saito, N. ; Nagashima, G. ; Nowak, Thaddeus ; Ruetzler, C. A. ; Klatzo, I. / Cardiac arrest-induced complete cerebral ischaemia in the rat : Dynamics of postischaemic in vivo calcium uptake and protein synthesis. In: Neurological Research. 1993 ; Vol. 15, No. 4. pp. 253-263.
@article{cc0abe45ed6b4a1aa964d646749e3f6e,
title = "Cardiac arrest-induced complete cerebral ischaemia in the rat: Dynamics of postischaemic in vivo calcium uptake and protein synthesis",
abstract = "Dynamics of pathological changes following cardiac arrest induced cerebral ischaemia were related to the findings of double tracer autoradiography of 45Ca and 3H leucine uptake as respective indicators of ischaemic injury and metabolic disturbance. Abnormal calcium accumulation, determined by 45Ca uptake, was related to injured but still living neurons and to reactive glial elements. 45Ca autoradiography confirmed a high sensitivity to neuronal injury of the nucleus reticularis thalami (NRT), hippocampal CA1 pyramidal layer, inferior colliculus, ventral thalamic nucleus (VTN), caudate nucleus, and parietal cortex. 3H leucine incorporation revealed that an initially widespread inhibition of protein synthesis was followed by its considerable recovery. Observations concerning hippocampal CA1 sector and VTN suggested that a significant degree of protein synthesis, maintained at the late stage after postischaemic recovery, was related to survival and regeneration of neurons and not to the presence of glial elements.",
author = "G. Mies and K. Kawai and N. Saito and G. Nagashima and Thaddeus Nowak and Ruetzler, {C. A.} and I. Klatzo",
year = "1993",
month = "1",
day = "1",
doi = "10.1080/01616412.1993.11740145",
language = "English (US)",
volume = "15",
pages = "253--263",
journal = "Neurological Research",
issn = "0161-6412",
publisher = "Maney Publishing",
number = "4",

}

TY - JOUR

T1 - Cardiac arrest-induced complete cerebral ischaemia in the rat

T2 - Dynamics of postischaemic in vivo calcium uptake and protein synthesis

AU - Mies, G.

AU - Kawai, K.

AU - Saito, N.

AU - Nagashima, G.

AU - Nowak, Thaddeus

AU - Ruetzler, C. A.

AU - Klatzo, I.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Dynamics of pathological changes following cardiac arrest induced cerebral ischaemia were related to the findings of double tracer autoradiography of 45Ca and 3H leucine uptake as respective indicators of ischaemic injury and metabolic disturbance. Abnormal calcium accumulation, determined by 45Ca uptake, was related to injured but still living neurons and to reactive glial elements. 45Ca autoradiography confirmed a high sensitivity to neuronal injury of the nucleus reticularis thalami (NRT), hippocampal CA1 pyramidal layer, inferior colliculus, ventral thalamic nucleus (VTN), caudate nucleus, and parietal cortex. 3H leucine incorporation revealed that an initially widespread inhibition of protein synthesis was followed by its considerable recovery. Observations concerning hippocampal CA1 sector and VTN suggested that a significant degree of protein synthesis, maintained at the late stage after postischaemic recovery, was related to survival and regeneration of neurons and not to the presence of glial elements.

AB - Dynamics of pathological changes following cardiac arrest induced cerebral ischaemia were related to the findings of double tracer autoradiography of 45Ca and 3H leucine uptake as respective indicators of ischaemic injury and metabolic disturbance. Abnormal calcium accumulation, determined by 45Ca uptake, was related to injured but still living neurons and to reactive glial elements. 45Ca autoradiography confirmed a high sensitivity to neuronal injury of the nucleus reticularis thalami (NRT), hippocampal CA1 pyramidal layer, inferior colliculus, ventral thalamic nucleus (VTN), caudate nucleus, and parietal cortex. 3H leucine incorporation revealed that an initially widespread inhibition of protein synthesis was followed by its considerable recovery. Observations concerning hippocampal CA1 sector and VTN suggested that a significant degree of protein synthesis, maintained at the late stage after postischaemic recovery, was related to survival and regeneration of neurons and not to the presence of glial elements.

UR - http://www.scopus.com/inward/record.url?scp=0027292752&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027292752&partnerID=8YFLogxK

U2 - 10.1080/01616412.1993.11740145

DO - 10.1080/01616412.1993.11740145

M3 - Article

VL - 15

SP - 253

EP - 263

JO - Neurological Research

JF - Neurological Research

SN - 0161-6412

IS - 4

ER -