Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals

Peter J. O'Brien, Gregory W. Dameron, Mary Lee Beck, Yujian Kang, B. Kipp Erickson, Tana H. Di Battista, Kim E. Miller, Keith N. Jackson, Scott Mittelstadt

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

A reliable serum assay that can discriminate between cardiac and skeletal muscle injury is not available for diagnostic use in laboratory animals. We tested and supported the hypotheses that serum cardiac troponin T (cTnT) was widely applicable in laboratory animals as a biomarker of cardiac injury arising from various causes; that it increased in proportion to severity of cardiac injury; and that it was more cardiospecific than creatine kinase (CK) or lactate dehydrogenase (LD) isozyme activities. In canine and rat models of myocardial infarction, cTnT concentration increased 1,000- to 10,000-fold and was highly correlated with infarct size within 3 h of injury. Serum CK and LD isozymes were substantially less effective biomarkers and, in contrast to cTnT, were ineffective markers in the presence of moderate skeletal muscle injury, with resulting serum CK activity >5,000 U/L. Using these animal models, and mouse and ferret models, we also showed cTnT to be an effective biomarker in doxorubicin cardiotoxicosis, traumatic injury, ischemia, and cardiac puncture. Reference range serum concentrations for all species were at the detection limit of the assay, except those for mice, in which they were slightly increased, possibly because mice were used to generate assay monoclonal antibodies. We conclude that cTnT is a powerful biomarker in laboratory animals for the sensitive and specific detection of cardiac injury arising from various causes.

Original languageEnglish (US)
Pages (from-to)486-495
Number of pages10
JournalLaboratory Animal Science
Volume47
Issue number5
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

troponin T
Troponin T
Laboratory Animals
Biomarkers
laboratory animals
biomarkers
Animals
creatine kinase
Creatine Kinase
Wounds and Injuries
Assays
lactate dehydrogenase
L-Lactate Dehydrogenase
Isoenzymes
Serum
Muscle
skeletal muscle
isozymes
mice
assays

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • veterinary(all)

Cite this

O'Brien, P. J., Dameron, G. W., Beck, M. L., Kang, Y., Kipp Erickson, B., Di Battista, T. H., ... Mittelstadt, S. (1997). Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals. Laboratory Animal Science, 47(5), 486-495.

Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals. / O'Brien, Peter J.; Dameron, Gregory W.; Beck, Mary Lee; Kang, Yujian; Kipp Erickson, B.; Di Battista, Tana H.; Miller, Kim E.; Jackson, Keith N.; Mittelstadt, Scott.

In: Laboratory Animal Science, Vol. 47, No. 5, 01.12.1997, p. 486-495.

Research output: Contribution to journalArticle

O'Brien, PJ, Dameron, GW, Beck, ML, Kang, Y, Kipp Erickson, B, Di Battista, TH, Miller, KE, Jackson, KN & Mittelstadt, S 1997, 'Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals', Laboratory Animal Science, vol. 47, no. 5, pp. 486-495.
O'Brien PJ, Dameron GW, Beck ML, Kang Y, Kipp Erickson B, Di Battista TH et al. Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals. Laboratory Animal Science. 1997 Dec 1;47(5):486-495.
O'Brien, Peter J. ; Dameron, Gregory W. ; Beck, Mary Lee ; Kang, Yujian ; Kipp Erickson, B. ; Di Battista, Tana H. ; Miller, Kim E. ; Jackson, Keith N. ; Mittelstadt, Scott. / Cardiac Troponin T is a Sensitive, Specific Biomarker of Cardiac Injury in Laboratory Animals. In: Laboratory Animal Science. 1997 ; Vol. 47, No. 5. pp. 486-495.
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