Caspases and osteogenic markers—in vitro screening of inhibition impact

Eva Adamova, Eva Janeckova, Karel Kleparnik, Eva Matalova

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Caspases, well-known players in apoptosis or inflammation, appear to have roles also in other processes such as cell differentiation. Caspase-3, in particular, was recently demonstrated to have non-apoptotic functions in osteogenesis. However, the molecular pathways involved are not yet known. Therefore, we used osteogenic PCR arrays to provide a comprehensive screening of possible interactions of caspases in general and specifically of caspase-3 in osteogenic networks. Embryonic micromass cultures derived from mouse forelimbs were established and pharmacological fluoromethylketone (FMK) inhibitors applied. Alterations were observed in expression of several genes after caspase inhibition (Bmp1, Bmp5, Bmp6, Col10a1, Col2a1, Comp, Egf, Fgfr2, Gli1, Igf1, Nog, Phex, Sox9, Spp1). The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies.

Original languageEnglish (US)
Pages (from-to)144-148
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume52
Issue number2
DOIs
StatePublished - Feb 1 2016

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Caspases
Caspase 3
Forelimb
Osteogenesis
Cell Differentiation
Pharmacology
Apoptosis
Inflammation
Gene Expression
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Caspases and osteogenic markers—in vitro screening of inhibition impact. / Adamova, Eva; Janeckova, Eva; Kleparnik, Karel; Matalova, Eva.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 52, No. 2, 01.02.2016, p. 144-148.

Research output: Contribution to journalArticle

Adamova, Eva ; Janeckova, Eva ; Kleparnik, Karel ; Matalova, Eva. / Caspases and osteogenic markers—in vitro screening of inhibition impact. In: In Vitro Cellular and Developmental Biology - Animal. 2016 ; Vol. 52, No. 2. pp. 144-148.
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