Catalase-overexpressing transgenic mouse heart is resistant to ischemia- reperfusion injury

Guangqiu Li, Yan Chen, Jack T. Saari, Yujian Kang

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Myocardial ischemia-reperfusion injury is at least partially mediated by oxygen-derived free radicals. Catalase is a major enzyme involved in the detoxification of hydrogen peroxide. The activity of catalase in the heart is very low, which may be a factor responsible for the high sensitivity of the heart to ischemia-reperfusion injury. The present study was undertaken to determine whether elevation of catalase specifically in the heart of transgenic mice can provide protection against ischemia-reperfusion injury. Hearts isolated from transgenic mice in which catalase in the heart was elevated ~60-fold higher than that in nontransgenic heart and from the non- transgenic littermates were subjected to 50 min of warm (37°C) zero-flow ischemia followed by 90 min reflow. Compared with nontransgenic controls, transgenic hearts showed significantly improved recovery of contractile force (75 vs. 25% at the end of 90 min reperfusion, P < 0.01). Efflux of creatine kinase was reduced by ~50%, and the zone of myocardial infarction as demarcated by triphenyltetrazolium at the end of reperfusion was reduced by ~40% in transgenic hearts compared with nontransgenic controls. These findings support the view that hydrogen peroxide is an important cause of ischemia-reperfusion damage and suggest that protection may be provided by elevation of catalase activity.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume273
Issue number3 42-3
StatePublished - Oct 13 1997

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Reperfusion Injury
Catalase
Transgenic Mice
Hydrogen Peroxide
Reperfusion
Ischemia
Myocardial Reperfusion Injury
Creatine Kinase
Free Radicals
Myocardial Ischemia
Myocardial Infarction
Oxygen
Enzymes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Catalase-overexpressing transgenic mouse heart is resistant to ischemia- reperfusion injury. / Li, Guangqiu; Chen, Yan; Saari, Jack T.; Kang, Yujian.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 273, No. 3 42-3, 13.10.1997.

Research output: Contribution to journalArticle

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