Catalytic activities of the human T-cell leukemia virus type II integrase

Mini Balakrishnan, Devon Zastrow, Colleen Jonsson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Despite the widespread nature of HTLV-II in New World populations and intravenous drug users, the enzymatic activities of the pol genes have not been reported. To ascertain the activity of the HTLV-II(G12) integrase (IN), the coding region was isolated and the encoded protein was purified, using nickel-affinity chromatography, to greater than 90% homogeneity. HTLV-II(G12) IN proved active on HTLV-II(G12) and HIV-1 integration and disintegration substrates. Distinct differences in requirements for enzyme concentration for 3'-processing, strand-transfer, and disintegration reactions were observed. Catalysis of integration reactions occurred in the presence of either Mn2+ or Mg2+, although strand-transfer activity preferred Mn2+. In comparison, HTLV-II(G12) IN catalyzed disintegration reactions with almost 10-fold less protein, was not selective for Mn2+ or Mg2+, and tolerated higher NaCl concentrations than integration. HTLV-II(G12) IN was unable to catalyze the 'splicing' reaction, which suggests that this may not be an activity ubiquitous to all retroviral integrases.

Original languageEnglish (US)
Pages (from-to)77-86
Number of pages10
JournalVirology
Volume219
Issue number1
DOIs
StatePublished - May 1 1996
Externally publishedYes

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Human T-lymphotropic virus 2
Integrases
pol Genes
Drug Users
Nickel
Catalysis
Affinity Chromatography
HIV-1
Proteins
Enzymes
Population

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Catalytic activities of the human T-cell leukemia virus type II integrase. / Balakrishnan, Mini; Zastrow, Devon; Jonsson, Colleen.

In: Virology, Vol. 219, No. 1, 01.05.1996, p. 77-86.

Research output: Contribution to journalArticle

Balakrishnan, Mini ; Zastrow, Devon ; Jonsson, Colleen. / Catalytic activities of the human T-cell leukemia virus type II integrase. In: Virology. 1996 ; Vol. 219, No. 1. pp. 77-86.
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