Cationic liposomes enhance targeted delivery and expression of exogenous DNA mediated by N-terminal modified poly(l-lysine)-antibody conjugate in mouse lung endothelial cells

Vladimir S. Trubetskoy, Vladimir P. Torchilin, Stephen Kennel, Leaf Huang

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

A new and improved system for targeted gene delivery and expression is described. Transfection efficiency of N-terminal modified poly(l-lysine) (NPLL) conjugated with anti-thrombomodulin antibody 34A can be improved by adding to the system a lipophilic component, cationic liposomes. DNA, antibody conjugate and cationic liposomes form a ternary electrostatic complex which preserves the ability to bind specifically to the target cells. At the same time the addition of liposomes enhance the specific transfection efficiency of antibody-polylysine/DNA binary complex by 10 to 20-fold in mouse lung endothelial cells in culture.

Original languageEnglish (US)
Pages (from-to)311-313
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1131
Issue number3
DOIs
StatePublished - Jul 15 1992
Externally publishedYes

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Endothelial cells
Liposomes
Lysine
Endothelial Cells
Lung
Transfection
Antibodies
DNA
Thrombomodulin
Polylysine
Static Electricity
Cell culture
Anti-Idiotypic Antibodies
Electrostatics
Cell Culture Techniques
Genes
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Cationic liposomes enhance targeted delivery and expression of exogenous DNA mediated by N-terminal modified poly(l-lysine)-antibody conjugate in mouse lung endothelial cells. / Trubetskoy, Vladimir S.; Torchilin, Vladimir P.; Kennel, Stephen; Huang, Leaf.

In: BBA - Gene Structure and Expression, Vol. 1131, No. 3, 15.07.1992, p. 311-313.

Research output: Contribution to journalArticle

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