Caudal cuneate nucleus projection to the direct thalamic relay to motor cortex in cat

an electrophysiological and anatomical study

Yasuhiko Tamai, Robert Waters, Hiroshi Asanuma

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20% solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer10. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.

Original languageEnglish (US)
Pages (from-to)360-364
Number of pages5
JournalBrain Research
Volume323
Issue number2
DOIs
StatePublished - Dec 10 1984
Externally publishedYes

Fingerprint

Motor Cortex
Cats
Neurons
Electrodes
Ventral Thalamic Nuclei
Brain

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

Caudal cuneate nucleus projection to the direct thalamic relay to motor cortex in cat : an electrophysiological and anatomical study. / Tamai, Yasuhiko; Waters, Robert; Asanuma, Hiroshi.

In: Brain Research, Vol. 323, No. 2, 10.12.1984, p. 360-364.

Research output: Contribution to journalArticle

@article{9dbbc7985498436cb08473b4c798a1d7,
title = "Caudal cuneate nucleus projection to the direct thalamic relay to motor cortex in cat: an electrophysiological and anatomical study",
abstract = "The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20{\%} solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer10. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.",
author = "Yasuhiko Tamai and Robert Waters and Hiroshi Asanuma",
year = "1984",
month = "12",
day = "10",
doi = "10.1016/0006-8993(84)90314-7",
language = "English (US)",
volume = "323",
pages = "360--364",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Caudal cuneate nucleus projection to the direct thalamic relay to motor cortex in cat

T2 - an electrophysiological and anatomical study

AU - Tamai, Yasuhiko

AU - Waters, Robert

AU - Asanuma, Hiroshi

PY - 1984/12/10

Y1 - 1984/12/10

N2 - The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20% solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer10. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.

AB - The input to the border region between the ventrolateral nucleus (VL) and ventroposterolateral nucleus (VPL) of the thalamus, VL-VPL, was studied in cats using a combined electrophysiological and anatomical technique. Neurons within this border region receive somatic afferent input and project to a region of the motor cortex having similar receptive fields. In this study we asked the question whether neurons in the VL-VPL border receive input from the dorsal column nuclei (DCN). To answer this question we delivered intra-cortical microstimulation (ICMS) to the motor cortex while a second electrode inserted into the VL-VPL border, filled with a 20% solution of HRP dissolved in KCl, was used to record antidromically activated neurons. When an antidromically activated neuron was encountered and the neuron responded to natural peripheral stimulation, HRP was iontophoretically injected through the recording electrode. After a 48-72 h survival time, cats were sacrificed, and the brain tissue processed according to the method of Hardy and Heimer10. Labeled cell bodies were found in the caudal cuneate nucleus (CCN) in all injected animals. These results suggested that neurons in CCN project to cells in VL-VPL which in turn project to the motor cortex.

UR - http://www.scopus.com/inward/record.url?scp=0021733617&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021733617&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(84)90314-7

DO - 10.1016/0006-8993(84)90314-7

M3 - Article

VL - 323

SP - 360

EP - 364

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -