Cdc42/N-WASP and Rac1/WAVE2 required for LPA-induced migration of rat ascites hepatoma cells

Ayako Uchiyama, Gabor Tigyi, Kimiko Murakami-Murofushi

Research output: Contribution to journalArticle

Abstract

Lysophosphatidic acid (LPA) has been shown to stimulate transcellular migration of MM1 rat ascites hepatoma cells through transient activation of the Rho/ROCK pathway. Here we report that LPA also induces transient inactivation of Cdc42 and Racl, both of which are required for transmigration of MM1 cells. Cdc42-induced shape change is a required step for LPA-induced MM1 cell migration. We provide evidence that MM1 cells transmigrate the mesothelial cell monolayer through activation of not only previously reported Rho/ROCK pathway but also Rac/WAVE2 and Cdc42/N-WASP pathways.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalCytologia
Volume75
Issue number2
DOIs
StatePublished - Jun 2010

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ascites
hepatoma
Ascites
Hepatocellular Carcinoma
acids
rats
cells
cell movement
Cell Movement
inactivation
lysophosphatidic acid

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Genetics
  • Plant Science
  • Animal Science and Zoology

Cite this

Cdc42/N-WASP and Rac1/WAVE2 required for LPA-induced migration of rat ascites hepatoma cells. / Uchiyama, Ayako; Tigyi, Gabor; Murakami-Murofushi, Kimiko.

In: Cytologia, Vol. 75, No. 2, 06.2010, p. 195-201.

Research output: Contribution to journalArticle

Uchiyama, Ayako ; Tigyi, Gabor ; Murakami-Murofushi, Kimiko. / Cdc42/N-WASP and Rac1/WAVE2 required for LPA-induced migration of rat ascites hepatoma cells. In: Cytologia. 2010 ; Vol. 75, No. 2. pp. 195-201.
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