CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury

Chengwei Duan, Yonghua Liu, Lu Lu, Rixin Cai, Huaqing Xue, Xingxing Mao, Chen Chen, Rong Qian, Dongmei Zhang, Aiguo Shen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cyclin-dependent kinases (CDKs) are perceived as the engine that drives cell cycle progression whereas cyclins are considered to be the gears that are changed to aid the transition between cycle phases. CDK14 is a cdc2-related serine/threonine protein kinase and plays an important role in normal cell cycle progression. However, its distribution and function in the central nervous system (CNS) lesion remain unclear. In this study, we mainly investigated the protein expression and cellular localization of CDK14 during spinal cord injury (SCI). Western blot analysis revealed that the expression of CDK14 was gradually increased and reached a peak at 3 days after SCI. The expression of CDK14 was further analyzed by immunohistochemistry. Double immunofluorescence staining showed that CDK14 was co-expressed prominent in astrocytes. Co-localization CDK14/proliferating cell nuclear antigen (PCNA) were detected in glial cells. cyclin Y, which can interact with CDK14, was detected that had same expression trend was consistent with CDK14 Western blot results in SCI. Double-immunofluorescence staining indicated that CDK14 co-expressed with cyclin Y in some cells. Co-immunoprecipitation had been showed that CDK14 could interact with cyclin Y after acute SCI. Taken together, these data suggested that both CDK14 and cyclin Y may play important roles in spinal cord pathophysiology.

Original languageEnglish (US)
Pages (from-to)571-579
Number of pages9
JournalJournal of Molecular Neuroscience
Volume57
Issue number4
DOIs
StatePublished - Dec 1 2015
Externally publishedYes

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Cyclins
Gliosis
Spinal Cord Injuries
Fluorescent Antibody Technique
Cell Cycle
Western Blotting
Cyclin-Dependent Kinase 2
Staining and Labeling
Cyclin-Dependent Kinases
Protein-Serine-Threonine Kinases
Proliferating Cell Nuclear Antigen
Immunoprecipitation
Neuroglia
Astrocytes
Spinal Cord
Central Nervous System
Immunohistochemistry
Proteins

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

Cite this

CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury. / Duan, Chengwei; Liu, Yonghua; Lu, Lu; Cai, Rixin; Xue, Huaqing; Mao, Xingxing; Chen, Chen; Qian, Rong; Zhang, Dongmei; Shen, Aiguo.

In: Journal of Molecular Neuroscience, Vol. 57, No. 4, 01.12.2015, p. 571-579.

Research output: Contribution to journalArticle

Duan, Chengwei ; Liu, Yonghua ; Lu, Lu ; Cai, Rixin ; Xue, Huaqing ; Mao, Xingxing ; Chen, Chen ; Qian, Rong ; Zhang, Dongmei ; Shen, Aiguo. / CDK14 Contributes to Reactive Gliosis via Interaction with Cyclin Y in Rat Model of Spinal Cord Injury. In: Journal of Molecular Neuroscience. 2015 ; Vol. 57, No. 4. pp. 571-579.
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