cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP

Silva H. Hanissian, Umar Akbar, Bin Teng, Zorica Janjetovic, Anne Hoffmann, Johann K. Hitzler, Norman Iscove, Kristin Hamre, Xiaoping Du, Yiai Tong, Suraj Mukatira, Jon H. Robertson, Stephan W. Morris

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Myelodysplasia/acute myeloid leukemia (MDS/AML) is characterized by a t(3;5)(q25.1;q34) chromosomal translocation that forms a fusion gene between nucleophosmin (NPM) and MDS/myeloid leukemia factor 1 (MLF1). We identified a novel protein, MLF1-interacting protein (MLF1IP), that specifically associates with MLF1 by yeast two-hybrid analysis and in pulldown assays, and colocalizes with it in both the nuclei and cytoplasm of cells. The MLF1IP gene locus is at chromosome 4q35.1 and is composed of 14 exons spanning 75.8 kb of genomic DNA. The MLF1IP cDNA encodes a 46-kDa protein that contains two bipartite and two classical nuclear localization signals, two nuclear receptor-binding motifs (LXXLL), two leucine zippers, two PEST residues and several potential phosphorylation sites. MLF1IP transcripts are expressed in a variety of tissues (e.g. fetal liver, bone marrow, thymus and testis). MLF1IP appears to be a lineage-specific gene whose expression is confined exclusively to the CFU-E erythroid precursor cells, but not in mature erythrocytes. These observations, together with previous data demonstrating a role for MLF1 in suppressing red cell maturation, suggest a possible role for MLF1IP and MLF1 deregulation in the genesis of erythroleukemias.

Original languageEnglish (US)
Pages (from-to)3700-3707
Number of pages8
JournalOncogene
Volume23
Issue number20
DOIs
StatePublished - Apr 29 2004

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Myeloid Leukemia
Organism Cloning
Complementary DNA
Genes
Proteins
Erythroid Precursor Cells
Leucine Zippers
Nuclear Localization Signals
Leukemia, Erythroblastic, Acute
Genetic Translocation
Gene Fusion
Cytoplasmic and Nuclear Receptors
Cell Nucleus
Acute Myeloid Leukemia
Thymus Gland
Testis
Exons
Cytoplasm
Fetus
Chromosomes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Hanissian, S. H., Akbar, U., Teng, B., Janjetovic, Z., Hoffmann, A., Hitzler, J. K., ... Morris, S. W. (2004). cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. Oncogene, 23(20), 3700-3707. https://doi.org/10.1038/sj.onc.1207448

cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. / Hanissian, Silva H.; Akbar, Umar; Teng, Bin; Janjetovic, Zorica; Hoffmann, Anne; Hitzler, Johann K.; Iscove, Norman; Hamre, Kristin; Du, Xiaoping; Tong, Yiai; Mukatira, Suraj; Robertson, Jon H.; Morris, Stephan W.

In: Oncogene, Vol. 23, No. 20, 29.04.2004, p. 3700-3707.

Research output: Contribution to journalArticle

Hanissian, SH, Akbar, U, Teng, B, Janjetovic, Z, Hoffmann, A, Hitzler, JK, Iscove, N, Hamre, K, Du, X, Tong, Y, Mukatira, S, Robertson, JH & Morris, SW 2004, 'cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP', Oncogene, vol. 23, no. 20, pp. 3700-3707. https://doi.org/10.1038/sj.onc.1207448
Hanissian SH, Akbar U, Teng B, Janjetovic Z, Hoffmann A, Hitzler JK et al. cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. Oncogene. 2004 Apr 29;23(20):3700-3707. https://doi.org/10.1038/sj.onc.1207448
Hanissian, Silva H. ; Akbar, Umar ; Teng, Bin ; Janjetovic, Zorica ; Hoffmann, Anne ; Hitzler, Johann K. ; Iscove, Norman ; Hamre, Kristin ; Du, Xiaoping ; Tong, Yiai ; Mukatira, Suraj ; Robertson, Jon H. ; Morris, Stephan W. / cDNA cloning and characterization of a novel gene encoding the MLF1-interacting protein MLF1IP. In: Oncogene. 2004 ; Vol. 23, No. 20. pp. 3700-3707.
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