Cell Survival within Pulp and Periodontal Constructs

Matthew Gebhardt, Peter E. Murray, Kenneth N. Namerow, Sergio Kuttler, Franklin Garcia-Godoy

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The purpose of this study was to measure cell survival and degradation within tissue-engineered dental constructs. Dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PLSCs) were seeded on three types of tissue engineering scaffolds: a synthetic open-cell D,D-L,L-polylactic acid (polymer) scaffold, a bovine collagen scaffold (collagen), and a calcium phosphate bioceramic (calcium phosphate) scaffold. The dental pulp and periodontal constructs (n = 144) were maintained in cell culture for between 3 and 14 days. The cell survival and degradation within the constructs were measured using histologic criteria. The DPSC and PLSC survival was optimal in the polymer and collagen constructs but not the calcium phosphate constructs, especially over longer time periods. These in vitro results suggest that both the polymer and collagen scaffolds and the DPSCs and PLSCs can be combined to create pulp and periodontal constructs for use in future regenerative dental treatments.

Original languageEnglish (US)
Pages (from-to)63-66
Number of pages4
JournalJournal of Endodontics
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

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Dental Pulp
Cell Survival
Stem Cells
Periodontal Ligament
Collagen
Polymers
Tooth
Tissue Scaffolds
Artificial Cells
Tissue Engineering
Cell Culture Techniques
calcium phosphate

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Cell Survival within Pulp and Periodontal Constructs. / Gebhardt, Matthew; Murray, Peter E.; Namerow, Kenneth N.; Kuttler, Sergio; Garcia-Godoy, Franklin.

In: Journal of Endodontics, Vol. 35, No. 1, 01.01.2009, p. 63-66.

Research output: Contribution to journalArticle

Gebhardt, Matthew ; Murray, Peter E. ; Namerow, Kenneth N. ; Kuttler, Sergio ; Garcia-Godoy, Franklin. / Cell Survival within Pulp and Periodontal Constructs. In: Journal of Endodontics. 2009 ; Vol. 35, No. 1. pp. 63-66.
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