Cell transplant techniques

Engraftment Detection of Cells

Robert A. Fisher, Valeria Mas

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

The use of isolated human hepatocyte infusions to treat human disease will require safe, acceptable, reliable, and reproducible measures of engraftment and function of the donor liver cell. Cell transplant for inborn errors of hepatic metabolism can be followed by measuring the specific protein missing from the recipient, expressed by the transplanted unmodified donor hepatocytes expressing the genes in question. This chapter will focus on the clinical techniques successful in identifying the engraftment and function of donor human hepatocytes when no specific identifiable genes are expressed by donor hepatocytes in acute and chronic liver diseases treated by cell infusion. Radiolabeling and dye labeling techniques, DNA typing of HLA class I alleles, soluble class I HLA ELISA, real-time quantitative PCR techniques including short tandem repeats analysis will be detailed and critiqued.

Original languageEnglish (US)
Title of host publicationHepatocyte Transplantation
Subtitle of host publicationMethods and Protocols
PublisherHumana Press
Pages97-105
Number of pages9
ISBN (Print)9781588298836
DOIs
StatePublished - Jan 1 2009

Publication series

NameMethods in Molecular Biology
Volume481
ISSN (Print)1064-3745

Fingerprint

Hepatocytes
Transplants
Inborn Errors Metabolism
DNA Fingerprinting
Liver
Microsatellite Repeats
Genes
Liver Diseases
Real-Time Polymerase Chain Reaction
Chronic Disease
Coloring Agents
Enzyme-Linked Immunosorbent Assay
Alleles
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Fisher, R. A., & Mas, V. (2009). Cell transplant techniques: Engraftment Detection of Cells. In Hepatocyte Transplantation: Methods and Protocols (pp. 97-105). (Methods in Molecular Biology; Vol. 481). Humana Press. https://doi.org/10.1007/978-1-59745-201-4_9

Cell transplant techniques : Engraftment Detection of Cells. / Fisher, Robert A.; Mas, Valeria.

Hepatocyte Transplantation: Methods and Protocols. Humana Press, 2009. p. 97-105 (Methods in Molecular Biology; Vol. 481).

Research output: Chapter in Book/Report/Conference proceedingChapter

Fisher, RA & Mas, V 2009, Cell transplant techniques: Engraftment Detection of Cells. in Hepatocyte Transplantation: Methods and Protocols. Methods in Molecular Biology, vol. 481, Humana Press, pp. 97-105. https://doi.org/10.1007/978-1-59745-201-4_9
Fisher RA, Mas V. Cell transplant techniques: Engraftment Detection of Cells. In Hepatocyte Transplantation: Methods and Protocols. Humana Press. 2009. p. 97-105. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-201-4_9
Fisher, Robert A. ; Mas, Valeria. / Cell transplant techniques : Engraftment Detection of Cells. Hepatocyte Transplantation: Methods and Protocols. Humana Press, 2009. pp. 97-105 (Methods in Molecular Biology).
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