Cellular proliferation in the nasal septal cartilage of juvenile minipigs

Ayman Al Dayeh, Susan W. Herring

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The growth of the nasal septal cartilage is believed to be a driving force of midfacial growth. Cellular proliferation is an important contributor to growth of the cartilage, but this factor has been rarely investigated. The current study was undertaken to assess the proliferation and cellular density in the septal cartilage of fast-growing juvenile minipigs. Six minipigs averaging 4.4±1months old were injected with 5′-bromo-2′-deoxyuridine (BrdU), a thymidine analog, 24h before death. The septal cartilage was sectioned in the coronal plane and reacted for BrdU. The proliferative index (number of BrdU-positive chondrocytes/total number of chondrocytes) and cellular density (number of cells mm-2) of various locations of the septum were measured and compared in order to determine overall proliferation rate and whether regional variations in proliferative activity and cellular density are present. To provide a time perspective to the problem of midfacial growth, the lengths of the nasal bone and the palate were measured in a collection of 61 dry skulls of minipigs aged 1-8months. Results showed that the septal chondrocytes were proliferating at a surprisingly high rate (~21%). The proliferative index was higher in the ventral and middle compared with the dorsal locations, and in the central cartilage compared with the perichondrium. No difference in proliferative index was found between the anterior and posterior parts of the septum. Cellular density was higher in the perichondrium than in the central cartilage. Within the central cartilage there was a trend for higher cellular density anteriorly. In conclusion, the rapidly growing midface of juvenile minipigs is associated with a high rate of septal proliferation, especially in the ventral half of the cartilage.

Original languageEnglish (US)
Pages (from-to)604-613
Number of pages10
JournalJournal of Anatomy
Volume225
Issue number6
DOIs
StatePublished - Jan 1 2014

Fingerprint

Nasal Cartilages
Miniature Swine
cartilage
Cartilage
cell proliferation
Cell Proliferation
Bromodeoxyuridine
Chondrocytes
chondrocytes
Growth
Cell Count
skull
Nasal Bone
bone
Palate
palate
Skull
Thymidine
thymidine
Intercellular Signaling Peptides and Proteins

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Cellular proliferation in the nasal septal cartilage of juvenile minipigs. / Al Dayeh, Ayman; Herring, Susan W.

In: Journal of Anatomy, Vol. 225, No. 6, 01.01.2014, p. 604-613.

Research output: Contribution to journalArticle

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