Ceramide induces interleukin 6 gene expression in human fibroblasts

Stanley J.E. Laulederkind, Alicja Bielawska, Rajendra Raghow, Yusuf A. Hannun, Leslie R. Ballou

Research output: Contribution to journalArticle

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Abstract

We previously reported that ceramide, the immediate product of sphingomyelin hydrolysis, increases in response to interleukin (IL)-I-and plays a role in modulating IL-l--mediated prostaglandin E2 production and cyclooxygenase gene expression in human fibroblasts (Ballou, L. K., C. P. Chao, M. A. Holness, S. C. Barker, and R. Raghow. 1992.J. Biol. Chem. 267:20044-20050). Here we describe the effects of ceramide in another IL-β-mediated process in these cells, the induction of Ib6 production. We found that submicromolar concentrations of C2-ceramide induced IL-6 gene expression and protein production as effectively as IL-β. Both D-erythro-Czceramide (a cell-permeable analogue of natural ceramide) and D-threo-C2-ceramide were potent inducers of IL-6 production, while neither t isomer of ceramide was effective. Compared with IL-l--induced Ib6 production, cells treated with ceramide or exogenous sphingomyelinase induced 82 and 50% of maximal IL-β-induced Ib6 levels by 6 h, respectively; by 24 h all three treatments induced similar levels of IL-6 production. Ceramide-induced IL-6 messenger KNA could be detected within 1 h of treatment and reached maximal levels by 24 h. These findings suggest that ceramide may play a role in the regulation of Ib6 gene expression.

Original languageEnglish (US)
Pages (from-to)599-604
Number of pages6
JournalJournal of Experimental Medicine
Volume182
Issue number2
DOIs
StatePublished - Aug 1 1995

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Ceramides
Interleukin-6
Interleukins
Fibroblasts
Gene Expression
Sphingomyelin Phosphodiesterase
Sphingomyelins
Gene Expression Regulation
Prostaglandin-Endoperoxide Synthases
Interleukin-1
Dinoprostone
Hydrolysis
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Laulederkind, S. J. E., Bielawska, A., Raghow, R., Hannun, Y. A., & Ballou, L. R. (1995). Ceramide induces interleukin 6 gene expression in human fibroblasts. Journal of Experimental Medicine, 182(2), 599-604. https://doi.org/10.1084/jem.182.2.599

Ceramide induces interleukin 6 gene expression in human fibroblasts. / Laulederkind, Stanley J.E.; Bielawska, Alicja; Raghow, Rajendra; Hannun, Yusuf A.; Ballou, Leslie R.

In: Journal of Experimental Medicine, Vol. 182, No. 2, 01.08.1995, p. 599-604.

Research output: Contribution to journalArticle

Laulederkind, SJE, Bielawska, A, Raghow, R, Hannun, YA & Ballou, LR 1995, 'Ceramide induces interleukin 6 gene expression in human fibroblasts', Journal of Experimental Medicine, vol. 182, no. 2, pp. 599-604. https://doi.org/10.1084/jem.182.2.599
Laulederkind, Stanley J.E. ; Bielawska, Alicja ; Raghow, Rajendra ; Hannun, Yusuf A. ; Ballou, Leslie R. / Ceramide induces interleukin 6 gene expression in human fibroblasts. In: Journal of Experimental Medicine. 1995 ; Vol. 182, No. 2. pp. 599-604.
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