Cerebral superoxide anion generation during seizures in newborn pigs

W. M. Armstead, R. Mirro, Charles Leffler, D. W. Busija

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Cerebral superoxide anion generation during bicuculline-induced seizures was measured in newborn pigs. Using two closed cranial windows inserted over the parietal cortices, superoxide dismutase (SOD)-inhibitable nitroblue tetrazolium (NBT) reduction was determined during 20 min of seizure activity induced by bicuculline, 5 mg/kg i.v. A modest increase in SOD-inhibitable NBT reduction was observed in piglets subjected to bicuculline-induced seizure activity (2.4 ± 0.6 pmol/mm2 in 20 min) when compared to control piglets (0.4 ± 0.3 pmol/mm2 in 20 min). Pretreatment with indomethacin (5 mg/kg i.v.) reduced SOD-inhibitable NBT reduction during seizures to the control level (0.5 ± 0.4 pmol/mm2 in 20 min). We conclude that small quantities of superoxide anion radical are produced by newborn pig brain during bicuculline-induced seizures and that cyclooxygenase metabolism of arachidonic acid appears to be a major source.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1989

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Superoxides
Bicuculline
Seizures
Swine
Nitroblue Tetrazolium
Superoxide Dismutase
Parietal Lobe
Prostaglandin-Endoperoxide Synthases
Indomethacin
Brain

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Cerebral superoxide anion generation during seizures in newborn pigs. / Armstead, W. M.; Mirro, R.; Leffler, Charles; Busija, D. W.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 9, No. 2, 01.01.1989, p. 175-179.

Research output: Contribution to journalArticle

Armstead, W. M. ; Mirro, R. ; Leffler, Charles ; Busija, D. W. / Cerebral superoxide anion generation during seizures in newborn pigs. In: Journal of Cerebral Blood Flow and Metabolism. 1989 ; Vol. 9, No. 2. pp. 175-179.
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