Change in surface hardness of enamel by a cola drink and a CPP-ACP paste

Daranee Versluis, A. Huang, M. D. Ericson, S. Poolthong

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Objectives: This in vitro study used surface microhardness to evaluate whether a paste containing casein phosphopeptide amorphous calcium phosphate (CPP-ACP) can reharden tooth enamel softened by a cola drink, and how different saliva-substitute solutions affect the enamel hardness. Methods: Twenty-four bovine incisors, each tooth consisting of treatment and control halves, were immersed in a cola drink (Coke®) for 8 min, then placed under a 0.4 mL/min drip with various saliva-substitute solutions. The saliva-substitute solutions were: saliva-like solution (SLS) with 1 ppm fluoride, SLS without fluoride, and Biotene® mouthwash. CPP-ACP paste was applied to the treatment halves for 3 min at 0, 8, 24, and 36 h. Knoop microhardness measurements were performed at baseline, after the cola drink immersion, and after 24 and 48 h contact with saliva-substitute solution. Results: Enamel hardness significantly decreased after immersion in cola drink (ANOVA, p < 0.05). After contact with saliva-like solutions for 48 h, those treated with CPP-ACP paste were significantly harder than those untreated regardless of the presence of 1 ppm fluoride in the saliva-like solution (ANOVA, p < 0.05). Biotene® mouthwash significantly softened the enamel surface (ANOVA, p < 0.05). Two-way ANOVA showed significant effects of the CPP-ACP paste application and types of saliva-substitute solutions on the changes in surface hardness of the softened enamel at a significance level of 0.05. Conclusion: The application of CPP-ACP paste with continuous replenishment of saliva-like solution for 48 h significantly hardened enamel softened by a cola drink. Biotene® mouthwash softened enamel surface after 48 h contact.

Original languageEnglish (US)
Pages (from-to)74-79
Number of pages6
JournalJournal of Dentistry
Volume36
Issue number1
DOIs
StatePublished - Jan 1 2008

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Phosphopeptides
Hardness
Dental Enamel
Ointments
Caseins
Saliva
Mouthwashes
Analysis of Variance
Fluorides
Immersion
Tooth
amorphous calcium phosphate
Coke
Incisor

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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Change in surface hardness of enamel by a cola drink and a CPP-ACP paste. / Versluis, Daranee; Huang, A.; Ericson, M. D.; Poolthong, S.

In: Journal of Dentistry, Vol. 36, No. 1, 01.01.2008, p. 74-79.

Research output: Contribution to journalArticle

Versluis, Daranee ; Huang, A. ; Ericson, M. D. ; Poolthong, S. / Change in surface hardness of enamel by a cola drink and a CPP-ACP paste. In: Journal of Dentistry. 2008 ; Vol. 36, No. 1. pp. 74-79.
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