Changes in Brain Energy Metabolism and Protein Synthesis Following Transient Bilateral Ischemia in the Gerbil

Thaddeus Nowak, Richard L. Fried, W. David Lust, Janet V. Passonneau

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Abstract: The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min; in vitro incorporation activity of brain supernatants was reduced to approximately 10% of control at 10 or 30 min recirculation, showed slight recovery at 60 min, and returned to 60% of control activity by 4 h. Protein synthesis activity was indistinguishable from control at 24 h. One minute of ischemia produced no detectable effect on protein synthesis measured after 30 min reperfusion; longer periods of ischemia resulted in progressive inhibition, with 5 min producing the maximal effect. Pentobarbital (50 mg/kg) increased by 1–2 min the threshold ischemic duration required to produce a given effect. Whereas most metabolites recovered quickly following 5 min ischemia, glycogen showed a delayed recovery comparable to that seen for protein synthesis. These results are discussed in relation to possible mechanisms for the coordinate regulation of brain energy metabolism and protein synthesis. An improved method for the fluorimetric measurement of guanine nucleotides is described.

Original languageEnglish (US)
Pages (from-to)487-494
Number of pages8
JournalJournal of Neurochemistry
Volume44
Issue number2
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Gerbillinae
Energy Metabolism
Brain
Ischemia
Proteins
Metabolites
Recovery
Guanine Nucleotides
Pentobarbital
Glycogen
Reperfusion

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Changes in Brain Energy Metabolism and Protein Synthesis Following Transient Bilateral Ischemia in the Gerbil. / Nowak, Thaddeus; Fried, Richard L.; Lust, W. David; Passonneau, Janet V.

In: Journal of Neurochemistry, Vol. 44, No. 2, 01.01.1985, p. 487-494.

Research output: Contribution to journalArticle

Nowak, Thaddeus ; Fried, Richard L. ; Lust, W. David ; Passonneau, Janet V. / Changes in Brain Energy Metabolism and Protein Synthesis Following Transient Bilateral Ischemia in the Gerbil. In: Journal of Neurochemistry. 1985 ; Vol. 44, No. 2. pp. 487-494.
@article{16d99d366c4d4aca9c146ff27ccc59f2,
title = "Changes in Brain Energy Metabolism and Protein Synthesis Following Transient Bilateral Ischemia in the Gerbil",
abstract = "Abstract: The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min; in vitro incorporation activity of brain supernatants was reduced to approximately 10{\%} of control at 10 or 30 min recirculation, showed slight recovery at 60 min, and returned to 60{\%} of control activity by 4 h. Protein synthesis activity was indistinguishable from control at 24 h. One minute of ischemia produced no detectable effect on protein synthesis measured after 30 min reperfusion; longer periods of ischemia resulted in progressive inhibition, with 5 min producing the maximal effect. Pentobarbital (50 mg/kg) increased by 1–2 min the threshold ischemic duration required to produce a given effect. Whereas most metabolites recovered quickly following 5 min ischemia, glycogen showed a delayed recovery comparable to that seen for protein synthesis. These results are discussed in relation to possible mechanisms for the coordinate regulation of brain energy metabolism and protein synthesis. An improved method for the fluorimetric measurement of guanine nucleotides is described.",
author = "Thaddeus Nowak and Fried, {Richard L.} and Lust, {W. David} and Passonneau, {Janet V.}",
year = "1985",
month = "1",
day = "1",
doi = "10.1111/j.1471-4159.1985.tb05440.x",
language = "English (US)",
volume = "44",
pages = "487--494",
journal = "Journal of Neurochemistry",
issn = "0022-3042",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Changes in Brain Energy Metabolism and Protein Synthesis Following Transient Bilateral Ischemia in the Gerbil

AU - Nowak, Thaddeus

AU - Fried, Richard L.

AU - Lust, W. David

AU - Passonneau, Janet V.

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Abstract: The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min; in vitro incorporation activity of brain supernatants was reduced to approximately 10% of control at 10 or 30 min recirculation, showed slight recovery at 60 min, and returned to 60% of control activity by 4 h. Protein synthesis activity was indistinguishable from control at 24 h. One minute of ischemia produced no detectable effect on protein synthesis measured after 30 min reperfusion; longer periods of ischemia resulted in progressive inhibition, with 5 min producing the maximal effect. Pentobarbital (50 mg/kg) increased by 1–2 min the threshold ischemic duration required to produce a given effect. Whereas most metabolites recovered quickly following 5 min ischemia, glycogen showed a delayed recovery comparable to that seen for protein synthesis. These results are discussed in relation to possible mechanisms for the coordinate regulation of brain energy metabolism and protein synthesis. An improved method for the fluorimetric measurement of guanine nucleotides is described.

AB - Abstract: The time course of the reduction in brain protein synthesis following transient bilateral ischemia in the gerbil was characterized and compared with changes in a number of metabolites related to brain energy metabolism. The recovery of brain protein synthesis was similar following ischemic periods of 5, 10, or 20 min; in vitro incorporation activity of brain supernatants was reduced to approximately 10% of control at 10 or 30 min recirculation, showed slight recovery at 60 min, and returned to 60% of control activity by 4 h. Protein synthesis activity was indistinguishable from control at 24 h. One minute of ischemia produced no detectable effect on protein synthesis measured after 30 min reperfusion; longer periods of ischemia resulted in progressive inhibition, with 5 min producing the maximal effect. Pentobarbital (50 mg/kg) increased by 1–2 min the threshold ischemic duration required to produce a given effect. Whereas most metabolites recovered quickly following 5 min ischemia, glycogen showed a delayed recovery comparable to that seen for protein synthesis. These results are discussed in relation to possible mechanisms for the coordinate regulation of brain energy metabolism and protein synthesis. An improved method for the fluorimetric measurement of guanine nucleotides is described.

UR - http://www.scopus.com/inward/record.url?scp=0021968895&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021968895&partnerID=8YFLogxK

U2 - 10.1111/j.1471-4159.1985.tb05440.x

DO - 10.1111/j.1471-4159.1985.tb05440.x

M3 - Article

VL - 44

SP - 487

EP - 494

JO - Journal of Neurochemistry

JF - Journal of Neurochemistry

SN - 0022-3042

IS - 2

ER -