Changes in liver inorganic pyrophosphate content during ethanol metabolism.

George Cook, M. T. King, R. L. Veech

Research output: Contribution to journalArticle

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Abstract

The injection of ethanol (70 mmol/kg body weight) into male Wistar rats fed ad libitum caused an increase within 15 min in the liver inorganic pyrophosphate (PPi) content from 0.012 +/- 0.001 mumol/g (wet weight) to 0.029 +/- 0.001 mumol/g. The injection of acetate (20 mmol/kg body weight) increased the liver PPi to 0.157 +/- 0.075 mumol/g in 15 min. The alcohol dehydrogenase inhibitor 4-methylpyrazole blocked the accumulation of acetate and the increase in PPi was prevented. Disulfiram, an inhibitor of aldehyde dehydrogenase, did not prevent changes in redox state, but the accumulation of acetate in the liver was decreased and the increase in liver PPi content was diminished (0.019 +/- 0.003 mumol/g). These data suggest that the increase in PPi observed after injection of ethanol may be due to the activation of the acetate produced during ethanol and acetaldehyde oxidation.

Original languageEnglish (US)
Pages (from-to)433-440
Number of pages8
JournalAdvances in Experimental Medicine and Biology
Volume132
StatePublished - 1980
Externally publishedYes

Fingerprint

Metabolism
Liver
Acetates
Ethanol
Injections
Body Weight
Disulfiram
Aldehyde Dehydrogenase
Acetaldehyde
Alcohol Dehydrogenase
Oxidation-Reduction
Wistar Rats
Rats
Chemical activation
Weights and Measures
Oxidation
diphosphoric acid

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Changes in liver inorganic pyrophosphate content during ethanol metabolism. / Cook, George; King, M. T.; Veech, R. L.

In: Advances in Experimental Medicine and Biology, Vol. 132, 1980, p. 433-440.

Research output: Contribution to journalArticle

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