Characterization of a new hemoprotein in the yeast Saccharomyces cerevisiae

Geppo Sartori, Laura Aldegheri, Gabriella Mazzotta, Gerolamo Lanfranchi, Helene Tournu, Alistair J.P. Brown, Giovanna Carignani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The Saccharomyces cerevisiae gene YNL234w encodes a 426-amino acid-long protein that shares significant similarities with the globin family. Compared with known globins from unicellular organisms, the Yn1234wp polypeptide is characterized by an unusual structure. In this protein, a central putative heme-binding domain of about 140 amino acids is flanked by two sequences of about 160 and 120 amino acids, respectively, which share no similarity with known polypeptides. Northern analysis indicates that YNL234w transcription is very low in cells grown under normal aerobic conditions but is induced by oxygen-limited growth conditions and by other stress conditions such as glucose repression, heat shock osmotic stress, and nitrogen starvation. However, the deletion of the gene had no detectable effect on yeast growth. The Yn1234wp polypeptide has been expressed in Escherichia coli, and the hemoprotein nature of the recombinant protein was demonstrated by heme staining after SDS/polyacrylamide gel electrophoresis and spectroscopic analysis. Our data indicate that purified recombinant Yn1234wp possesses a noncovalently bound heme molecule that is predominantly found in a low spin form.

Original languageEnglish (US)
Pages (from-to)5032-5037
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number8
DOIs
StatePublished - Feb 19 1999

Fingerprint

Heme
Yeast
Saccharomyces cerevisiae
Globins
Yeasts
Amino Acids
Peptides
Genes
Spectroscopic analysis
Osmotic Pressure
Gene Deletion
Transcription
Growth
Starvation
Electrophoresis
Recombinant Proteins
Escherichia coli
Polyacrylamide Gel Electrophoresis
Shock
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Sartori, G., Aldegheri, L., Mazzotta, G., Lanfranchi, G., Tournu, H., Brown, A. J. P., & Carignani, G. (1999). Characterization of a new hemoprotein in the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry, 274(8), 5032-5037. https://doi.org/10.1074/jbc.274.8.5032

Characterization of a new hemoprotein in the yeast Saccharomyces cerevisiae. / Sartori, Geppo; Aldegheri, Laura; Mazzotta, Gabriella; Lanfranchi, Gerolamo; Tournu, Helene; Brown, Alistair J.P.; Carignani, Giovanna.

In: Journal of Biological Chemistry, Vol. 274, No. 8, 19.02.1999, p. 5032-5037.

Research output: Contribution to journalArticle

Sartori, G, Aldegheri, L, Mazzotta, G, Lanfranchi, G, Tournu, H, Brown, AJP & Carignani, G 1999, 'Characterization of a new hemoprotein in the yeast Saccharomyces cerevisiae', Journal of Biological Chemistry, vol. 274, no. 8, pp. 5032-5037. https://doi.org/10.1074/jbc.274.8.5032
Sartori, Geppo ; Aldegheri, Laura ; Mazzotta, Gabriella ; Lanfranchi, Gerolamo ; Tournu, Helene ; Brown, Alistair J.P. ; Carignani, Giovanna. / Characterization of a new hemoprotein in the yeast Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 8. pp. 5032-5037.
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