Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line

Shuji Ozaki, Dennis Wolfenbarger, Mary DeBram-Hart, Sivadasan Kanangat, Deborah T. Weiss, Alan Solomon

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A new human monoclonal plasma cell line, designated UTMC-2, was established from the pleural effusion of a patient with immunoglobulin (Ig)A(κ)-related multiple myeloma. The cultured cells were Epstein-Barr virus-negative and exhibited the morphological and ultrastructural features characteristic of plasma cells. Immunohistochemical analyses revealed the presence of cytoplasmic IgA(κ) as well as the plasma cell-associated surface antigens CD38 and CD56. Other B-cell markers, including CD10, CD19, CD20, and HLA-DR, were absent. The UTMC-2 cells were interleukin (IL)-6 responsive: Co-culture with IL-6 increased IgA(κ) synthesis and cell proliferation in a dose-dependent manner. In contrast, an IL-6 antisense oligonucleotide had an opposite effect. Although the UTMC-2 cells expressed IL-6 mRNA (as demonstrated by reverse transcriptase-polymerase chain reaction (RT-PCR)) and contained IL-6, the concentration of this cytokine in cell culture supernatants was less than that detectable by the enzyme-linked immunosorbent assay (ELISA) employed (i.e. < 3 pg/ml). Further, cell growth was not inhibited by polyclonal or monoclonal anti-IL-6 antibodies. Flow cytometric analysis revealed that IL-6 receptors present on the surface of the UTMC-2 cells were not saturated with endogenous IL-6. Taken together, these results indicate that, in this human plasma cell line, IL-6 functions uniquely in an intracellular autocrine fashion to enhance Ig synthesis and cell growth. In this respect, the UTMC-2 cells represent a novel resource for further study of the role of IL-6 in the pathogenesis of multiple myeloma.

Original languageEnglish (US)
Pages (from-to)2207-2213
Number of pages7
JournalLeukemia
Volume8
Issue number12
StatePublished - Jan 1 1994

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Plasma Cells
Interleukin-6
Cell Line
Immunoglobulin A
Multiple Myeloma
Interleukin-6 Receptors
Antisense Oligonucleotides
HLA-DR Antigens
Pleural Effusion
Surface Antigens
Growth
Coculture Techniques
Reverse Transcriptase Polymerase Chain Reaction
Human Herpesvirus 4
Immunoglobulins
Cultured Cells
B-Lymphocytes
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Ozaki, S., Wolfenbarger, D., DeBram-Hart, M., Kanangat, S., Weiss, D. T., & Solomon, A. (1994). Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line. Leukemia, 8(12), 2207-2213.

Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line. / Ozaki, Shuji; Wolfenbarger, Dennis; DeBram-Hart, Mary; Kanangat, Sivadasan; Weiss, Deborah T.; Solomon, Alan.

In: Leukemia, Vol. 8, No. 12, 01.01.1994, p. 2207-2213.

Research output: Contribution to journalArticle

Ozaki, S, Wolfenbarger, D, DeBram-Hart, M, Kanangat, S, Weiss, DT & Solomon, A 1994, 'Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line', Leukemia, vol. 8, no. 12, pp. 2207-2213.
Ozaki S, Wolfenbarger D, DeBram-Hart M, Kanangat S, Weiss DT, Solomon A. Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line. Leukemia. 1994 Jan 1;8(12):2207-2213.
Ozaki, Shuji ; Wolfenbarger, Dennis ; DeBram-Hart, Mary ; Kanangat, Sivadasan ; Weiss, Deborah T. ; Solomon, Alan. / Characterization of a novel interleukin-6 autocrine-dependent human plasma cell line. In: Leukemia. 1994 ; Vol. 8, No. 12. pp. 2207-2213.
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