Characterization of a novel small RNA encoded by dictyostelium discoideum mitochondrial DNA

Min Pi, T. Morio, H. Urushihara, Y. Tanaka

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study, we analyzed a mitochondrial small (ms) RNA in Dictylostelium discoideum, which is 129 nucleotides long and has a GC content of only 22.5%. In the mitochondrial DNA, a single-copy gene (msr) for the ms RNA was located downstream of the gene for large-subunit rRNA. The location of msr was similar to that of the 5S rRNA gene in prokaryotes and chloroplasts, but clearly different from that in mitochondria of plants, liverwort and the chlorophycean alga Prototheca wikerhamii, in which small-subunit rRNA and 5S rRNA genes are closely linked. The primary sequence of ms RNA showed low homology with mitochondrial 5S rRNA from plants, liverwort and the chlorophycean alga, but the proposed secondary structure of ms RNA was similar to that of cytoplasmic 5S rRNA. In addition, ms RNA showed a highly conserved GAAC sequence in the same loop as in common 5S rRNA. However, ms RNA was detected mainly in the mitochondrial 25,000 x g supernatant fraction which was devoid of ribosomes. It is possible that ms RNA is an evolutionary derivative of mitochondrial 5S rRNA.

Original languageEnglish (US)
Pages (from-to)124-131
Number of pages8
JournalMolecular and General Genetics
Volume257
Issue number2
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

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Dictyostelium
Mitochondrial DNA
RNA
Hepatophyta
rRNA Genes
Prototheca
Conserved Sequence
Base Composition
Chloroplasts
mitochondrial RNA
Ribosomes
Genes
Mitochondria
Nucleotides

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Characterization of a novel small RNA encoded by dictyostelium discoideum mitochondrial DNA. / Pi, Min; Morio, T.; Urushihara, H.; Tanaka, Y.

In: Molecular and General Genetics, Vol. 257, No. 2, 01.01.1998, p. 124-131.

Research output: Contribution to journalArticle

Pi, Min ; Morio, T. ; Urushihara, H. ; Tanaka, Y. / Characterization of a novel small RNA encoded by dictyostelium discoideum mitochondrial DNA. In: Molecular and General Genetics. 1998 ; Vol. 257, No. 2. pp. 124-131.
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