Characterization of a Tumor Cell Surface Protein with Heterologous Antisera to a Spontaneous BALB/c Lung Carcinoma

Research output: Contribution to journalArticle

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Abstract

Line 1, a spontaneous alveolar carcinoma from a BALB/c mouse, is highly metastatic and weakly antigenic in the syngeneic host. Sera and enriched antibody preparations were made specific for line 1 cells by in vitro and in vivo absorptions. By lactoperoxidase-catalyzed radioiodination of cell surface protein followed by precipitation with specific antibodies, a protein with a molecular weight of about 180,000 (designated TSP-180) was identified that was present on line 1 cells but not on normal lung cells or Moloney sarcoma tumor cells. Studies of competition for immune precipitation of TSP-180 by unlabeled protein preparations from various sources indicate that (a) TSP-180 is present in tumor cells of various culture passage levels, (b) tumor cells grown i.m. also contain TSP-180, and (c) normal lung proteins compete weakly, and only at very high protein concentrations. A protein similar to TSP-180 was detected on Madison 109 carcinoma and on three other lung carcinomas adapted to culture. Experiments with specific antisera show that TSP-180 is not lactoperoxidase, fetal bovine serum protein, large external transformation-sensitive protein, or an antigen related to murine leukemia virus proteins.

Original languageEnglish (US)
Pages (from-to)2934-2939
Number of pages6
JournalCancer Research
Volume39
Issue number8
StatePublished - 1979
Externally publishedYes

Fingerprint

Integrin alpha6beta4
Immune Sera
Membrane Proteins
Carcinoma
Lung
Neoplasms
Proteins
Lactoperoxidase
Bronchiolo-Alveolar Adenocarcinoma
Murine Leukemia Viruses
Antibodies
Immunoprecipitation
Sarcoma
Blood Proteins
Cell Culture Techniques
Molecular Weight
Antigens

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Oncology

Cite this

Characterization of a Tumor Cell Surface Protein with Heterologous Antisera to a Spontaneous BALB/c Lung Carcinoma. / Kennel, Stephen.

In: Cancer Research, Vol. 39, No. 8, 1979, p. 2934-2939.

Research output: Contribution to journalArticle

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abstract = "Line 1, a spontaneous alveolar carcinoma from a BALB/c mouse, is highly metastatic and weakly antigenic in the syngeneic host. Sera and enriched antibody preparations were made specific for line 1 cells by in vitro and in vivo absorptions. By lactoperoxidase-catalyzed radioiodination of cell surface protein followed by precipitation with specific antibodies, a protein with a molecular weight of about 180,000 (designated TSP-180) was identified that was present on line 1 cells but not on normal lung cells or Moloney sarcoma tumor cells. Studies of competition for immune precipitation of TSP-180 by unlabeled protein preparations from various sources indicate that (a) TSP-180 is present in tumor cells of various culture passage levels, (b) tumor cells grown i.m. also contain TSP-180, and (c) normal lung proteins compete weakly, and only at very high protein concentrations. A protein similar to TSP-180 was detected on Madison 109 carcinoma and on three other lung carcinomas adapted to culture. Experiments with specific antisera show that TSP-180 is not lactoperoxidase, fetal bovine serum protein, large external transformation-sensitive protein, or an antigen related to murine leukemia virus proteins.",
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N2 - Line 1, a spontaneous alveolar carcinoma from a BALB/c mouse, is highly metastatic and weakly antigenic in the syngeneic host. Sera and enriched antibody preparations were made specific for line 1 cells by in vitro and in vivo absorptions. By lactoperoxidase-catalyzed radioiodination of cell surface protein followed by precipitation with specific antibodies, a protein with a molecular weight of about 180,000 (designated TSP-180) was identified that was present on line 1 cells but not on normal lung cells or Moloney sarcoma tumor cells. Studies of competition for immune precipitation of TSP-180 by unlabeled protein preparations from various sources indicate that (a) TSP-180 is present in tumor cells of various culture passage levels, (b) tumor cells grown i.m. also contain TSP-180, and (c) normal lung proteins compete weakly, and only at very high protein concentrations. A protein similar to TSP-180 was detected on Madison 109 carcinoma and on three other lung carcinomas adapted to culture. Experiments with specific antisera show that TSP-180 is not lactoperoxidase, fetal bovine serum protein, large external transformation-sensitive protein, or an antigen related to murine leukemia virus proteins.

AB - Line 1, a spontaneous alveolar carcinoma from a BALB/c mouse, is highly metastatic and weakly antigenic in the syngeneic host. Sera and enriched antibody preparations were made specific for line 1 cells by in vitro and in vivo absorptions. By lactoperoxidase-catalyzed radioiodination of cell surface protein followed by precipitation with specific antibodies, a protein with a molecular weight of about 180,000 (designated TSP-180) was identified that was present on line 1 cells but not on normal lung cells or Moloney sarcoma tumor cells. Studies of competition for immune precipitation of TSP-180 by unlabeled protein preparations from various sources indicate that (a) TSP-180 is present in tumor cells of various culture passage levels, (b) tumor cells grown i.m. also contain TSP-180, and (c) normal lung proteins compete weakly, and only at very high protein concentrations. A protein similar to TSP-180 was detected on Madison 109 carcinoma and on three other lung carcinomas adapted to culture. Experiments with specific antisera show that TSP-180 is not lactoperoxidase, fetal bovine serum protein, large external transformation-sensitive protein, or an antigen related to murine leukemia virus proteins.

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