Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors

Influence of cyclin l isoforms on splice site selection

Pascal Loyer, Janeen H. Trembley, Jose A. Grenet, Adeline Busson, Anne Corlu, Wei Zhao, Mehmet Kocak, Vincent J. Kidd, Jill M. Lahti

Research output: Contribution to journalArticle

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Abstract

Although it has been reported that cyclin L1α and L2α proteins interact with CDK11p110, the nature of the cyclin L transcripts, the formation of complexes between the five cyclin L and the three CDK11 protein isoforms, and the influence of these complexes on splicing have not been thoroughly investigated. Here we report that cyclin L1 and L2 genes generate 14 mRNA variants encoding six cyclin L proteins, one of which has not been described previously. Using cyclin L gene-specific antibodies, we demonstrate expression of multiple endogenous cyclin L proteins in human cell lines and mouse tissues. Moreover, we characterize interactions between CDK11p110, mitosis-specific CDK11p58, and apoptosis-specific CDK11p46 with both cyclin Lα and -β proteins and the co-elution of these proteins following size exclusion chromatography. We further establish that CDK11p110 and associated cyclin Lα/β proteins localize to splicing factor compartments and nucleoplasm and interact with serine/arginine-rich proteins. Importantly, we also determine the effect of CDK11-cyclin L complexes on pre-mRNA splicing. Preincubation of nuclear extracts with purified cyclin Lα and -β isoforms depletes the extract of in vitro splicing activity. Ectopic expression of cyclin L1α, L1β, L2α, or L2β or active CDK11p110 individually enhances intracellular intron splicing activity, whereas expression of CDK11 p58/p46 or kinase-dead CDK11p110 represses splicing activity. Finally, we demonstrate that expression of cyclins Lα and -β and CDK11p110 strongly and differentially affects alternative splicing in vivo. Together, these data establish that CDK11p110 interacts physically and functionally with cyclin Lα and -β isoforms and SR proteins to regulate splicing.

Original languageEnglish (US)
Pages (from-to)7721-7732
Number of pages12
JournalJournal of Biological Chemistry
Volume283
Issue number12
DOIs
StatePublished - Mar 21 2008

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Site selection
Cyclins
Protein Isoforms
Proteins
RNA Splicing Factors
Genes
Size exclusion chromatography
RNA Precursors
Alternative Splicing
Mitosis
Introns
Serine
Gel Chromatography
Arginine

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors : Influence of cyclin l isoforms on splice site selection. / Loyer, Pascal; Trembley, Janeen H.; Grenet, Jose A.; Busson, Adeline; Corlu, Anne; Zhao, Wei; Kocak, Mehmet; Kidd, Vincent J.; Lahti, Jill M.

In: Journal of Biological Chemistry, Vol. 283, No. 12, 21.03.2008, p. 7721-7732.

Research output: Contribution to journalArticle

Loyer, Pascal ; Trembley, Janeen H. ; Grenet, Jose A. ; Busson, Adeline ; Corlu, Anne ; Zhao, Wei ; Kocak, Mehmet ; Kidd, Vincent J. ; Lahti, Jill M. / Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors : Influence of cyclin l isoforms on splice site selection. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 12. pp. 7721-7732.
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T1 - Characterization of cyclin L1 and L2 interactions with CDK11 and splicing factors

T2 - Influence of cyclin l isoforms on splice site selection

AU - Loyer, Pascal

AU - Trembley, Janeen H.

AU - Grenet, Jose A.

AU - Busson, Adeline

AU - Corlu, Anne

AU - Zhao, Wei

AU - Kocak, Mehmet

AU - Kidd, Vincent J.

AU - Lahti, Jill M.

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N2 - Although it has been reported that cyclin L1α and L2α proteins interact with CDK11p110, the nature of the cyclin L transcripts, the formation of complexes between the five cyclin L and the three CDK11 protein isoforms, and the influence of these complexes on splicing have not been thoroughly investigated. Here we report that cyclin L1 and L2 genes generate 14 mRNA variants encoding six cyclin L proteins, one of which has not been described previously. Using cyclin L gene-specific antibodies, we demonstrate expression of multiple endogenous cyclin L proteins in human cell lines and mouse tissues. Moreover, we characterize interactions between CDK11p110, mitosis-specific CDK11p58, and apoptosis-specific CDK11p46 with both cyclin Lα and -β proteins and the co-elution of these proteins following size exclusion chromatography. We further establish that CDK11p110 and associated cyclin Lα/β proteins localize to splicing factor compartments and nucleoplasm and interact with serine/arginine-rich proteins. Importantly, we also determine the effect of CDK11-cyclin L complexes on pre-mRNA splicing. Preincubation of nuclear extracts with purified cyclin Lα and -β isoforms depletes the extract of in vitro splicing activity. Ectopic expression of cyclin L1α, L1β, L2α, or L2β or active CDK11p110 individually enhances intracellular intron splicing activity, whereas expression of CDK11 p58/p46 or kinase-dead CDK11p110 represses splicing activity. Finally, we demonstrate that expression of cyclins Lα and -β and CDK11p110 strongly and differentially affects alternative splicing in vivo. Together, these data establish that CDK11p110 interacts physically and functionally with cyclin Lα and -β isoforms and SR proteins to regulate splicing.

AB - Although it has been reported that cyclin L1α and L2α proteins interact with CDK11p110, the nature of the cyclin L transcripts, the formation of complexes between the five cyclin L and the three CDK11 protein isoforms, and the influence of these complexes on splicing have not been thoroughly investigated. Here we report that cyclin L1 and L2 genes generate 14 mRNA variants encoding six cyclin L proteins, one of which has not been described previously. Using cyclin L gene-specific antibodies, we demonstrate expression of multiple endogenous cyclin L proteins in human cell lines and mouse tissues. Moreover, we characterize interactions between CDK11p110, mitosis-specific CDK11p58, and apoptosis-specific CDK11p46 with both cyclin Lα and -β proteins and the co-elution of these proteins following size exclusion chromatography. We further establish that CDK11p110 and associated cyclin Lα/β proteins localize to splicing factor compartments and nucleoplasm and interact with serine/arginine-rich proteins. Importantly, we also determine the effect of CDK11-cyclin L complexes on pre-mRNA splicing. Preincubation of nuclear extracts with purified cyclin Lα and -β isoforms depletes the extract of in vitro splicing activity. Ectopic expression of cyclin L1α, L1β, L2α, or L2β or active CDK11p110 individually enhances intracellular intron splicing activity, whereas expression of CDK11 p58/p46 or kinase-dead CDK11p110 represses splicing activity. Finally, we demonstrate that expression of cyclins Lα and -β and CDK11p110 strongly and differentially affects alternative splicing in vivo. Together, these data establish that CDK11p110 interacts physically and functionally with cyclin Lα and -β isoforms and SR proteins to regulate splicing.

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