Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen

Peter Klucar, Peter F. Barnes, Ying Kong, Buka Samten, Amy Tvinnereim, Ralf Spallek, Gerald T. Nepom, Mahavir Singh, Homayoun Shams

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

CD4+ T cells are believed to play a dominant role in human defenses against Mycobacterium tuberculosis through production of interferon (IFN)-γ, cytolytic T-cell (CTL) activity, and inhibition of intracellular mycobacterial growth. Most functional studies of CD4+ cells have used bulk T-cells that recognize crude mycobacterial antigens, and the functional capacity of individual human T cells is not well defined. We studied the functional capacity of human CD4+ T-cell clones that recognize a specific mycobacterial peptide. Clone B9 produced high concentrations of IFN-γ and exhibited potent CTL activity, whereas clone D3 produced IFN-γ but showed poor CTL activity. The CTL activity of clone B9 was inhibited by SrCl2 and concanamycin A but not by anti-Fas antibodies. Clone B9 also reduced the mycobacterial burden in dendritic cells by more than 90%, and this antimycobacterial activity was inhibited by SrCl2 and concanamycin A. We conclude that: (1) individual human peptide-specific CD4+ T-cell clones have differential capacity to produce Th1 cytokines and to lyse M tuberculosis-infected target cells; and (2) both granulysin and perforin contribute to the capacity of human CD4+ T-cells to lyse infected targets and to inhibit intracellular mycobacterial growth.

Original languageEnglish (US)
Pages (from-to)475-483
Number of pages9
JournalHuman Immunology
Volume69
Issue number8
DOIs
StatePublished - Aug 1 2008
Externally publishedYes

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Clone Cells
T-Lymphocytes
Peptides
Interferons
Perforin
Growth
Mycobacterium tuberculosis
Dendritic Cells
Anti-Idiotypic Antibodies
Tuberculosis
Cytokines
Antigens

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen. / Klucar, Peter; Barnes, Peter F.; Kong, Ying; Samten, Buka; Tvinnereim, Amy; Spallek, Ralf; Nepom, Gerald T.; Singh, Mahavir; Shams, Homayoun.

In: Human Immunology, Vol. 69, No. 8, 01.08.2008, p. 475-483.

Research output: Contribution to journalArticle

Klucar, P, Barnes, PF, Kong, Y, Samten, B, Tvinnereim, A, Spallek, R, Nepom, GT, Singh, M & Shams, H 2008, 'Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen', Human Immunology, vol. 69, no. 8, pp. 475-483. https://doi.org/10.1016/j.humimm.2008.05.008
Klucar, Peter ; Barnes, Peter F. ; Kong, Ying ; Samten, Buka ; Tvinnereim, Amy ; Spallek, Ralf ; Nepom, Gerald T. ; Singh, Mahavir ; Shams, Homayoun. / Characterization of effector functions of human peptide-specific CD4+ T-cell clones for an intracellular pathogen. In: Human Immunology. 2008 ; Vol. 69, No. 8. pp. 475-483.
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