Characterization of Fv-1 host range strains of murine retroviruses by titration and p30 protein characteristics

Raymond W. Tennant, James A. Otten, Arthur Brown, Wen K. Yang, Stephen Kennel

Research output: Contribution to journalArticle

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Abstract

A standardized, direct XC plaque assay was used to determine the titration hitness patterns of Fv-1 host range murine retroviruses obtained from various laboratories. The N- or B-tropic viruses were tested on a variety of cells with Fv-1nn, Fv-1bb, or Fv-1nb genotypes, and, with one exception, a discrete two-hit pattern was obtained on cells with the restrictive genotype (i.e., N-tropic virus on BALB/c, SIM·R, and B6C3F1 cells, and B-tropic virus on SME, SIM, and B6C3F1 cells). The single exception to the two-hit titration effect was a strain (TOR-B) which is poorly infectious for all cells, and which does not show a clear N- or B-tropism. In addition, it was possible to convert the two-hit pattern of another B-tropic virus (OR-B) to a one-hit curve by coinfection with an XC-negative N-tropic virus. Relative to SC-1 (Fv-1--) cells, it was possible to define three components of restriction of ecotropic virus infection of mouse cells: The first two components, the two-hit kinetics and 102- to 103-fold reduction in titer on restrictive cells, are Fv-1 determined; the third a decreased infectivity on all cells relative to SC-1 cells, is independent of Fv-1 genotype.

Original languageEnglish (US)
Pages (from-to)349-357
Number of pages9
JournalVirology
Volume99
Issue number2
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

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Host Specificity
Retroviridae
Proteins
Cercopithecine Herpesvirus 1
Genotype
Viruses
Tropism
Virus Diseases
Coinfection

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Characterization of Fv-1 host range strains of murine retroviruses by titration and p30 protein characteristics. / Tennant, Raymond W.; Otten, James A.; Brown, Arthur; Yang, Wen K.; Kennel, Stephen.

In: Virology, Vol. 99, No. 2, 01.01.1979, p. 349-357.

Research output: Contribution to journalArticle

Tennant, Raymond W. ; Otten, James A. ; Brown, Arthur ; Yang, Wen K. ; Kennel, Stephen. / Characterization of Fv-1 host range strains of murine retroviruses by titration and p30 protein characteristics. In: Virology. 1979 ; Vol. 99, No. 2. pp. 349-357.
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