Characterization of pseudotype VSV possessing HCV envelope proteins

Yoshiharu Matsuura, Hideki Tani, Kensuke Suzuki, Tomomi Kimura-Someya, Ryosuke Suzuki, Hideki Aizaki, Koji Ishii, Kohji Moriishi, Clinton S. Robison, Michael Whitt, Tatsuo Miyamura

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

The genome of hepatitis C virus (HCV) encodes two envelope glycoproteins (E1 and E2), which are thought to be responsible for receptor binding and membrane fusion resulting in virus penetration. To investigate cell surface determinants important for HCV infection, we used a recombinant vesicular stomatitis virus (VSV) in which the glycoprotein gene was replaced with a reporter gene encoding green fluorescent protein (GFP) and produced HCV-VSV pseudotypes possessing chimeric HCV E1 or E2 glycoproteins, either individually or together. The infectivity of the pseudotypes was determined by quantifying the number of cells expressing the GFP reporter gene. Pseudotypes that contained both of the chimeric E1 and E2 proteins exhibited 10-20 times higher infectivity on HepG2 cells than the viruses possessing either of the glycoproteins individually. These results indicated that both E1 and E2 envelope proteins are required for maximal infection by HCV. The infectivity of the pseudotype virus was not neutralized by anti-VSV polyclonal antibodies. Bovine lactoferrin specifically inhibited the infection of the pseudotype virus. Treatment of HepG2 cells with Pronase, heparinase, and heparitinase but not with phospholipase C and sodium periodate reduced the infectivity. Therefore, cell surface proteins and some glycosaminoglycans play an important role in binding or entry of HCV into susceptible cells. The pseudotype VSV possessing the chimeric HCV glycoproteins might offer an efficient tool for future research on cellular receptors for HCV and for the development of prophylactics and therapeutics for hepatitis C.

Original languageEnglish (US)
Pages (from-to)263-275
Number of pages13
JournalVirology
Volume286
Issue number2
DOIs
StatePublished - Aug 1 2001

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Viral Envelope Proteins
Vesicular Stomatitis
Hepacivirus
Viruses
Glycoproteins
Hep G2 Cells
Virus Diseases
heparitinsulfate lyase
Green Fluorescent Proteins
Reporter Genes
Heparin Lyase
Pronase
Membrane Fusion
Lactoferrin
Type C Phospholipases
Hepatitis C
Glycosaminoglycans
Membrane Proteins
Proteins
Cell Count

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Matsuura, Y., Tani, H., Suzuki, K., Kimura-Someya, T., Suzuki, R., Aizaki, H., ... Miyamura, T. (2001). Characterization of pseudotype VSV possessing HCV envelope proteins. Virology, 286(2), 263-275. https://doi.org/10.1006/viro.2001.0971

Characterization of pseudotype VSV possessing HCV envelope proteins. / Matsuura, Yoshiharu; Tani, Hideki; Suzuki, Kensuke; Kimura-Someya, Tomomi; Suzuki, Ryosuke; Aizaki, Hideki; Ishii, Koji; Moriishi, Kohji; Robison, Clinton S.; Whitt, Michael; Miyamura, Tatsuo.

In: Virology, Vol. 286, No. 2, 01.08.2001, p. 263-275.

Research output: Contribution to journalArticle

Matsuura, Y, Tani, H, Suzuki, K, Kimura-Someya, T, Suzuki, R, Aizaki, H, Ishii, K, Moriishi, K, Robison, CS, Whitt, M & Miyamura, T 2001, 'Characterization of pseudotype VSV possessing HCV envelope proteins', Virology, vol. 286, no. 2, pp. 263-275. https://doi.org/10.1006/viro.2001.0971
Matsuura Y, Tani H, Suzuki K, Kimura-Someya T, Suzuki R, Aizaki H et al. Characterization of pseudotype VSV possessing HCV envelope proteins. Virology. 2001 Aug 1;286(2):263-275. https://doi.org/10.1006/viro.2001.0971
Matsuura, Yoshiharu ; Tani, Hideki ; Suzuki, Kensuke ; Kimura-Someya, Tomomi ; Suzuki, Ryosuke ; Aizaki, Hideki ; Ishii, Koji ; Moriishi, Kohji ; Robison, Clinton S. ; Whitt, Michael ; Miyamura, Tatsuo. / Characterization of pseudotype VSV possessing HCV envelope proteins. In: Virology. 2001 ; Vol. 286, No. 2. pp. 263-275.
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