Characterization of the Apical Papilla and Its Residing Stem Cells from Human Immature Permanent Teeth: A Pilot Study

Wataru Sonoyama, Yi Liu, Takayoshi Yamaza, Rocky S. Tuan, Songlin Wang, Songtao Shi, George Huang

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Abstract

Mesenchymal stem cells (MSCs) have been isolated from the pulp tissue of permanent teeth (dental pulp stem cells or DPSCs) and deciduous teeth (stem cells from human exfoliated deciduous teeth). We recently discovered another type of MSCs in the apical papilla of human immature permanent teeth termed stem cells from the apical papilla (SCAP). Here, we further characterized the apical papilla tissue and stem cell properties of SCAP using histologic, immunohistochemical, and immunocytofluorescent analyses. We found that the apical papilla is distinctive to the pulp in terms of containing less cellular and vascular components than those in the pulp. Cells in the apical papilla proliferated 2- to 3-fold greater than those in the pulp in organ cultures. Both SCAP and DPSCs were as potent in osteo/dentinogenic differentiation as MSCs from bone marrows, whereas they were weaker in adipogenic potential. The immunophenotype of SCAP is similar to that of DPSCs on the osteo/dentinogenic and growth factor receptor gene profiles. Double-staining experiments showed that STRO-1 coexpressed with dentinogenic markers such as bone sialophosphoprotein, osteocalcin, and growth factors FGFR1 and TGFβRI in cultured SCAP. Additionally, SCAP express a wide variety of neurogenic markers such as nestin and neurofilament M upon stimulation with a neurogenic medium. We conclude that SCAP are similar to DPSCs but a distinct source of potent dental stem/progenitor cells. Their implications in root development and apexogenesis are discussed.

Original languageEnglish (US)
Pages (from-to)166-171
Number of pages6
JournalJournal of endodontics
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2008

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Tooth
Stem Cells
Mesenchymal Stromal Cells
Deciduous Tooth
Apexification
Nestin
Dental Pulp
Intermediate Filaments
Growth Factor Receptors
Organ Culture Techniques
Osteocalcin
Blood Vessels
Cultured Cells
Intercellular Signaling Peptides and Proteins
Bone Marrow
Staining and Labeling
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Characterization of the Apical Papilla and Its Residing Stem Cells from Human Immature Permanent Teeth : A Pilot Study. / Sonoyama, Wataru; Liu, Yi; Yamaza, Takayoshi; Tuan, Rocky S.; Wang, Songlin; Shi, Songtao; Huang, George.

In: Journal of endodontics, Vol. 34, No. 2, 01.02.2008, p. 166-171.

Research output: Contribution to journalArticle

Sonoyama, Wataru ; Liu, Yi ; Yamaza, Takayoshi ; Tuan, Rocky S. ; Wang, Songlin ; Shi, Songtao ; Huang, George. / Characterization of the Apical Papilla and Its Residing Stem Cells from Human Immature Permanent Teeth : A Pilot Study. In: Journal of endodontics. 2008 ; Vol. 34, No. 2. pp. 166-171.
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