Characterization of the Hantaan nucleocapsid protein-ribonucleic acid interaction

William Severson, Laurel Partin, Connie S. Schmaljohn, Colleen Jonsson

Research output: Contribution to journalArticle

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Abstract

The nucleocapsid (N) protein functions in hantavirus replication through its interactions with the vital genomic and antigenomic RNAs. To address the biological functions of the N protein, it was critical to first define this binding interaction. The dissociation constant, K(d), for the interaction of the Hantaan virus (HTNV) N protein and its genomic S segment (vRNA) was measured under several solution conditions. Overall, increasing the NaCl and Mg2+ in these binding reactions had little impact on the K(d). However, the HTNV N protein showed an enhanced specificity for HTNV vRNA as compared with the S segment open reading frame RNA or a nonviral RNA with increasing ionic strength and the presence of Mg2+. In contrast, the assembly of Sin Nombre virus N protein-HTNV vRNA complexes was inhibited by the presence of Mg2+ or an increase in the ionic strength. The K(d) values for HTNV and Sin Nombre virus N proteins were nearly identical for the S segment open reading frame RNA, showing weak affinity over several binding reaction conditions. Our data suggest a model in which specific recognition of the HTNV vRNA by the HTNV N protein resides in the noncoding regions of the HTNV vRNA.

Original languageEnglish (US)
Pages (from-to)33732-33739
Number of pages8
JournalJournal of Biological Chemistry
Volume274
Issue number47
DOIs
StatePublished - Nov 19 1999

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Hantaan virus
Nucleocapsid Proteins
Viruses
Sin Nombre virus
RNA
Osmolar Concentration
Open Reading Frames
Hantavirus
Ionic strength
Nucleocapsid
Proteins
Hantaan virus nucleocapsid protein

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of the Hantaan nucleocapsid protein-ribonucleic acid interaction. / Severson, William; Partin, Laurel; Schmaljohn, Connie S.; Jonsson, Colleen.

In: Journal of Biological Chemistry, Vol. 274, No. 47, 19.11.1999, p. 33732-33739.

Research output: Contribution to journalArticle

Severson, William ; Partin, Laurel ; Schmaljohn, Connie S. ; Jonsson, Colleen. / Characterization of the Hantaan nucleocapsid protein-ribonucleic acid interaction. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 47. pp. 33732-33739.
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