Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC-1 and CAPAN-1

B. Miszczuk-Jamska, M. Merten, O. Guy-Crotte, M. Amouric, F. Clemente, Robert Schoumacher, C. Figarella

Research output: Contribution to journalArticle

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Abstract

Proteins with trypsin-like immunoreactivity (first detected by a specific immunoenzymatic assay) were isolated from CAPAN-1 and CFPAC-1 cell culture-conditioned media by chromatography on an immunoadsorbent prepared with a polyclonal antibody directed against trypsin 1. The adsorbed proteins were devoid of free trypsin activity but trypsin activity was present after enterokinase activation demonstrating that the immunoreactive trypsin present in cell supernatants corresponds to trypsinogens. When characterised by Western blotting using a monoclonal antibody directed against human trypsin 1 two protein bands corresponding to trypsinogen 1 (23 kDa) and trypsinogen 2 (25 kDa) gave a positive reaction. These results demonstrate the presence of trypsinogens 1 and 2 in CAPAN-1 and CFPAC-1 cells and in their culture-conditioned media.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalFEBS Letters
Volume294
Issue number3
DOIs
StatePublished - Dec 9 1991

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Trypsinogen
Trypsin
Adenocarcinoma
Cells
Cell Line
Conditioned Culture Medium
Enteropeptidase
Immunosorbents
Proteins
Culture Media
Chromatography
Cell culture
Cell Culture Techniques
Western Blotting
Monoclonal Antibodies
Assays
Chemical activation
Antibodies

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC-1 and CAPAN-1. / Miszczuk-Jamska, B.; Merten, M.; Guy-Crotte, O.; Amouric, M.; Clemente, F.; Schoumacher, Robert; Figarella, C.

In: FEBS Letters, Vol. 294, No. 3, 09.12.1991, p. 175-178.

Research output: Contribution to journalArticle

Miszczuk-Jamska, B, Merten, M, Guy-Crotte, O, Amouric, M, Clemente, F, Schoumacher, R & Figarella, C 1991, 'Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC-1 and CAPAN-1', FEBS Letters, vol. 294, no. 3, pp. 175-178. https://doi.org/10.1016/0014-5793(91)80662-M
Miszczuk-Jamska, B. ; Merten, M. ; Guy-Crotte, O. ; Amouric, M. ; Clemente, F. ; Schoumacher, Robert ; Figarella, C. / Characterization of trypsinogens 1 and 2 in two human pancreatic adenocarcinoma cell lines; CFPAC-1 and CAPAN-1. In: FEBS Letters. 1991 ; Vol. 294, No. 3. pp. 175-178.
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AU - Clemente, F.

AU - Schoumacher, Robert

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