Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis

T. Hirose, R. A. Reife, G. N. Smith, R. M. Stevens, C. L. Mainardi, Karen Hasty

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Gelatin degrading matrix metalloproteinases in synovial fluid from 21 patients with inflammatory arthritis were shown to consist of two distinct gene products, 92 and 70 kDa gelatinases. The gelatinolytic activity of 92 kDa enzyme, which is released from stimulated neutrophils, was positively correlated to neutrophil count in the fluid. By contrast, 70 kDa molecule did not correlate with neutrophil cell count. Purification of these enzymes revealed they could degrade type XI collagen, a cartilage component resistant to interstitial collagenase. The elevated levels of 92 kDa gelatinase in rheumatoid arthritis samples compared to osteoarthritis suggest a role of this enzyme in cartilage destruction.

Original languageEnglish (US)
Pages (from-to)593-599
Number of pages7
JournalJournal of Rheumatology
Volume19
Issue number4
StatePublished - Jan 1 1992

Fingerprint

Gelatinases
Synovial Fluid
Collagenases
Arthritis
Neutrophils
Matrix Metalloproteinase 9
Cartilage
Collagen Type XI
Enzymes
Matrix Metalloproteinase 1
Gelatin
Matrix Metalloproteinases
Osteoarthritis
Rheumatoid Arthritis
Cell Count
Genes

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Immunology and Allergy
  • Immunology

Cite this

Hirose, T., Reife, R. A., Smith, G. N., Stevens, R. M., Mainardi, C. L., & Hasty, K. (1992). Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis. Journal of Rheumatology, 19(4), 593-599.

Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis. / Hirose, T.; Reife, R. A.; Smith, G. N.; Stevens, R. M.; Mainardi, C. L.; Hasty, Karen.

In: Journal of Rheumatology, Vol. 19, No. 4, 01.01.1992, p. 593-599.

Research output: Contribution to journalArticle

Hirose, T, Reife, RA, Smith, GN, Stevens, RM, Mainardi, CL & Hasty, K 1992, 'Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis', Journal of Rheumatology, vol. 19, no. 4, pp. 593-599.
Hirose, T. ; Reife, R. A. ; Smith, G. N. ; Stevens, R. M. ; Mainardi, C. L. ; Hasty, Karen. / Characterization of type V collagenase (gelatinase) in synovial fluid of patients with inflammatory arthritis. In: Journal of Rheumatology. 1992 ; Vol. 19, No. 4. pp. 593-599.
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