Characterizing sphingosine kinases and sphingosine 1-phosphate receptors in the mammalian eye and retina

Hunter Porter, Hui Qi, Nicole Prabhu, Richard Grambergs, Joel McRae, Blake Hopiavuori, Nawajes Mandal

Research output: Contribution to journalArticle

Abstract

Sphingosine 1-phosphate (S1P) signaling regulates numerous biological processes including neurogenesis, inflammation and neovascularization. However, little is known about the role of S1P signaling in the eye. In this study, we characterize two sphingosine kinases (SPHK1 and SPHK2), which phosphorylate sphingosine to S1P, and three S1P receptors (S1PR1, S1PR2 and S1PR3) in mouse and rat eyes. We evaluated sphingosine kinase and S1P receptor gene expression at the mRNA level in various rat tissues and rat retinas exposed to light-damage, whole mouse eyes, specific eye structures, and in developing retinas. Furthermore, we determined the localization of sphingosine kinases and S1P receptors in whole rat eyes by immunohistochemistry. Our results unveiled unique expression profiles for both sphingosine kinases and each receptor in ocular tissues. Furthermore, these kinases and S1P receptors are expressed in mammalian retinal cells and the expression of SPHK1, S1PR2 and S1PR3 increased immediately after light damage, which suggests a function in apoptosis and/or light stress responses in the eye. These findings have numerous implications for understanding the role of S1P signaling in the mechanisms of ocular diseases such as retinal inflammatory and degenerative diseases, neovascular eye diseases, glaucoma and corneal diseases.

Original languageEnglish (US)
Article number3885
JournalInternational journal of molecular sciences
Volume19
Issue number12
DOIs
StatePublished - Dec 1 2018

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Sphingosines
Lysosphingolipid Receptors
retina
Retina
Rats
phosphates
Phosphates
rats
Tissue
Eye Diseases
Sphingosine
Light
Gene expression
mice
Corneal Diseases
eye diseases
Biological Phenomena
Cells
glaucoma
Apoptosis

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Characterizing sphingosine kinases and sphingosine 1-phosphate receptors in the mammalian eye and retina. / Porter, Hunter; Qi, Hui; Prabhu, Nicole; Grambergs, Richard; McRae, Joel; Hopiavuori, Blake; Mandal, Nawajes.

In: International journal of molecular sciences, Vol. 19, No. 12, 3885, 01.12.2018.

Research output: Contribution to journalArticle

Porter, Hunter ; Qi, Hui ; Prabhu, Nicole ; Grambergs, Richard ; McRae, Joel ; Hopiavuori, Blake ; Mandal, Nawajes. / Characterizing sphingosine kinases and sphingosine 1-phosphate receptors in the mammalian eye and retina. In: International journal of molecular sciences. 2018 ; Vol. 19, No. 12.
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