Chemical modification of muscle protein in diabetes

Nadja Alt, James Carson, Nathan L. Alderson, Yuping Wang, Ryoji Nagai, Thomas Henle, Suzanne R. Thorpe, John W. Baynes

Research output: Contribution to journalArticle

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Abstract

Levels of glycation (fructose-lysine, FL) and advanced glycoxidation and lipoxidation end-products (AGE/ALEs) were measured in total skeletal (gastrocnemius) muscle and myofibril protein and compared to levels of the same compounds in insoluble skin collagen of control and diabetic rats. Levels of FL in total muscle and myofibril protein were 3-5% the level of FL in skin collagen. The AGE/ALEs, Nε-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine, were also significantly lower in total muscle and myofibril protein, ∼25% of levels in skin collagen. The newly described sulfhydryl AGE/ALE, S-(carboxymethyl)cysteine (CMC), was also measured in muscle; levels of CMC were comparable to those of CML and increased similarly in response to diabetes. Although FL and AGE/ALEs increased in muscle protein in diabetes, the relative increase was less than that seen in skin collagen. These data indicate that muscle protein is partially protected against the increase in both glycation and AGE/ALE formation in diabetes.

Original languageEnglish (US)
Pages (from-to)200-206
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume425
Issue number2
DOIs
StatePublished - May 15 2004

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Muscle Proteins
Chemical modification
Medical problems
Myofibrils
Skin
Carbocysteine
Collagen
Skeletal Muscle
Lysine
Muscle
Rats
Muscles
fructosyl-lysine
N(6)-carboxymethyllysine

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Alt, N., Carson, J., Alderson, N. L., Wang, Y., Nagai, R., Henle, T., ... Baynes, J. W. (2004). Chemical modification of muscle protein in diabetes. Archives of Biochemistry and Biophysics, 425(2), 200-206. https://doi.org/10.1016/j.abb.2004.03.012

Chemical modification of muscle protein in diabetes. / Alt, Nadja; Carson, James; Alderson, Nathan L.; Wang, Yuping; Nagai, Ryoji; Henle, Thomas; Thorpe, Suzanne R.; Baynes, John W.

In: Archives of Biochemistry and Biophysics, Vol. 425, No. 2, 15.05.2004, p. 200-206.

Research output: Contribution to journalArticle

Alt, N, Carson, J, Alderson, NL, Wang, Y, Nagai, R, Henle, T, Thorpe, SR & Baynes, JW 2004, 'Chemical modification of muscle protein in diabetes', Archives of Biochemistry and Biophysics, vol. 425, no. 2, pp. 200-206. https://doi.org/10.1016/j.abb.2004.03.012
Alt, Nadja ; Carson, James ; Alderson, Nathan L. ; Wang, Yuping ; Nagai, Ryoji ; Henle, Thomas ; Thorpe, Suzanne R. ; Baynes, John W. / Chemical modification of muscle protein in diabetes. In: Archives of Biochemistry and Biophysics. 2004 ; Vol. 425, No. 2. pp. 200-206.
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