Chemotaxis by various cytokeves of ia+ langerhans cells into the central cornea of mice

effect of interleukins 5 and 7

Research output: Contribution to journalArticle

Abstract

Purpose: Although under normal conditions it is devoid of Ia+ Langerhans cells (LC), a variety of stimuli, including several cytokines, have been shown to induce a centripetal migration of LC into the central cornea. Having previously shown Interleukins (IL)-3 and IL-4 to be lacking any such activity, we sought to determine whether IL-5 and IL-7 have such an effect upon these antigen presenting cells and, if so, how these cytokines might interplay in the known aspects of the chemotactic cascade. Methods: BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts of as much as 25.0 IU recombinant (r) murine (mu) IL-5 or 1.0 × 106 IU r mu IL-7 and 16 hours later evaluated by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) la+ LC peripherally, with none regularly found centrally or paracentrallv). Although intracorneal injection of r mu IL-5 failed to induce the migration of la' LC (0.25±0.46 BALB/c, 0.58±0.79 C57BL/6 and 0.83±0.83 C3H/HeJ), r mu IL-7 induced 29.88±2.42 BALB/c, 28.83±2.21 C57BL/6 and 27.67±2.02 C3H/HeJ Ia+ LC. The addition of cyclosporine A (CsA), interferon (DFN)-γ and transforming growth factor (TGF)-β abrogated this chemotactic effect while the addition of rapamycin(RAPA) did not. Thus, r mu IL-5 failed to induce the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied, while r mu IL-7 successfully induced the migration of these immunocompetant cells into the central corneas of these same murine strains (BALB/c: p=0.90; C57BL/6: p=0.99; C3H/HeJ: p=0.92). Conclusions: We propose that IL-7, but not IL-5, is chemotactic for Ia+ Langerhans cells.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997
Externally publishedYes

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Langerhans Cells
Interleukin-5
Chemotaxis
Cornea
Interleukin-7
Interleukin-16
Cytokines
Inbred C3H Mouse
Interleukin-3
Transforming Growth Factors
Antigen-Presenting Cells
Sirolimus
mouse interleukin-7
Interleukin-4
Interferons
Cyclosporine
Cell Movement
Staining and Labeling
Injections

All Science Journal Classification (ASJC) codes

  • Ophthalmology

Cite this

@article{8ad88340aa1e4d7c9d4b0da1db568719,
title = "Chemotaxis by various cytokeves of ia+ langerhans cells into the central cornea of mice: effect of interleukins 5 and 7",
abstract = "Purpose: Although under normal conditions it is devoid of Ia+ Langerhans cells (LC), a variety of stimuli, including several cytokines, have been shown to induce a centripetal migration of LC into the central cornea. Having previously shown Interleukins (IL)-3 and IL-4 to be lacking any such activity, we sought to determine whether IL-5 and IL-7 have such an effect upon these antigen presenting cells and, if so, how these cytokines might interplay in the known aspects of the chemotactic cascade. Methods: BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts of as much as 25.0 IU recombinant (r) murine (mu) IL-5 or 1.0 × 106 IU r mu IL-7 and 16 hours later evaluated by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) la+ LC peripherally, with none regularly found centrally or paracentrallv). Although intracorneal injection of r mu IL-5 failed to induce the migration of la' LC (0.25±0.46 BALB/c, 0.58±0.79 C57BL/6 and 0.83±0.83 C3H/HeJ), r mu IL-7 induced 29.88±2.42 BALB/c, 28.83±2.21 C57BL/6 and 27.67±2.02 C3H/HeJ Ia+ LC. The addition of cyclosporine A (CsA), interferon (DFN)-γ and transforming growth factor (TGF)-β abrogated this chemotactic effect while the addition of rapamycin(RAPA) did not. Thus, r mu IL-5 failed to induce the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied, while r mu IL-7 successfully induced the migration of these immunocompetant cells into the central corneas of these same murine strains (BALB/c: p=0.90; C57BL/6: p=0.99; C3H/HeJ: p=0.92). Conclusions: We propose that IL-7, but not IL-5, is chemotactic for Ia+ Langerhans cells.",
author = "Penny Asbell",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "4",

}

TY - JOUR

T1 - Chemotaxis by various cytokeves of ia+ langerhans cells into the central cornea of mice

T2 - effect of interleukins 5 and 7

AU - Asbell, Penny

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Purpose: Although under normal conditions it is devoid of Ia+ Langerhans cells (LC), a variety of stimuli, including several cytokines, have been shown to induce a centripetal migration of LC into the central cornea. Having previously shown Interleukins (IL)-3 and IL-4 to be lacking any such activity, we sought to determine whether IL-5 and IL-7 have such an effect upon these antigen presenting cells and, if so, how these cytokines might interplay in the known aspects of the chemotactic cascade. Methods: BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts of as much as 25.0 IU recombinant (r) murine (mu) IL-5 or 1.0 × 106 IU r mu IL-7 and 16 hours later evaluated by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) la+ LC peripherally, with none regularly found centrally or paracentrallv). Although intracorneal injection of r mu IL-5 failed to induce the migration of la' LC (0.25±0.46 BALB/c, 0.58±0.79 C57BL/6 and 0.83±0.83 C3H/HeJ), r mu IL-7 induced 29.88±2.42 BALB/c, 28.83±2.21 C57BL/6 and 27.67±2.02 C3H/HeJ Ia+ LC. The addition of cyclosporine A (CsA), interferon (DFN)-γ and transforming growth factor (TGF)-β abrogated this chemotactic effect while the addition of rapamycin(RAPA) did not. Thus, r mu IL-5 failed to induce the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied, while r mu IL-7 successfully induced the migration of these immunocompetant cells into the central corneas of these same murine strains (BALB/c: p=0.90; C57BL/6: p=0.99; C3H/HeJ: p=0.92). Conclusions: We propose that IL-7, but not IL-5, is chemotactic for Ia+ Langerhans cells.

AB - Purpose: Although under normal conditions it is devoid of Ia+ Langerhans cells (LC), a variety of stimuli, including several cytokines, have been shown to induce a centripetal migration of LC into the central cornea. Having previously shown Interleukins (IL)-3 and IL-4 to be lacking any such activity, we sought to determine whether IL-5 and IL-7 have such an effect upon these antigen presenting cells and, if so, how these cytokines might interplay in the known aspects of the chemotactic cascade. Methods: BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts of as much as 25.0 IU recombinant (r) murine (mu) IL-5 or 1.0 × 106 IU r mu IL-7 and 16 hours later evaluated by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) la+ LC peripherally, with none regularly found centrally or paracentrallv). Although intracorneal injection of r mu IL-5 failed to induce the migration of la' LC (0.25±0.46 BALB/c, 0.58±0.79 C57BL/6 and 0.83±0.83 C3H/HeJ), r mu IL-7 induced 29.88±2.42 BALB/c, 28.83±2.21 C57BL/6 and 27.67±2.02 C3H/HeJ Ia+ LC. The addition of cyclosporine A (CsA), interferon (DFN)-γ and transforming growth factor (TGF)-β abrogated this chemotactic effect while the addition of rapamycin(RAPA) did not. Thus, r mu IL-5 failed to induce the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied, while r mu IL-7 successfully induced the migration of these immunocompetant cells into the central corneas of these same murine strains (BALB/c: p=0.90; C57BL/6: p=0.99; C3H/HeJ: p=0.92). Conclusions: We propose that IL-7, but not IL-5, is chemotactic for Ia+ Langerhans cells.

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