Chemotaxis by various cytokines of Ia+ Langerhans cells into the central cornea of mice: Effect of interleukins 3 and 4

S. P. Epstein, Penny Asbell, G. Chamie, M. Davoodzadeh

Research output: Contribution to journalArticle

Abstract

Purpose: Although the central cornea under normal conditions is devoid of Ia+ Langerhans cells (LC), several cytokines have been shown to induce centripetal migration of LC. We sought to determine whether Interleukins (IL)-3 and IL-4 have an effect upon these antigen presenting cells. Methods: Applications of murine (mu) IL-3 are active in the concentration range of 0.1 to 10.0 ng/ml in vitro and 1.0 to 100.0 ng in vivo. Applications of mu IL-4 are active in the range of 0.5 to 40.0 ng/mL in vitro and 5.0 to 200.0 ng in vivo. BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts (max. 1.0μg) recombinant (r) murine (mu) IL-3 or 250 IU r mu IL-4 and evaluated 16 hours later by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas were found to contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) Ia+ LC peripherally, with none regularly found centrally or paracentrally. Intracorneal injection of r mu IL-3 induced the migration of 0.25±0.6 BALB/c, 0.42±0.5 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC into the cornea; while r mu IL-4 induced the migration of 0.25±0.6 BALB/c, 0.39±0.6 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC. Neither r mu IL-3 (BALB/c: p=0.90; C57BL/6: p=0.90; C3H/HeJ: p=0.92), nor r mu IL-4 (BALB/c: p=0.90; C57BL/6: p-0.99; C3H/HeJ: p=0.92) induced the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied. Conclusions: Prior exploration of the chemotactic cascade has demonstrated cytokine induction of Ia+ Langerhans cells into the central cornea. Unlike other cytokines (ie. IL-2, TNF-α, etc.), we propose that neither IL-3 nor IL-4 are chemotactic for Ia+ Langerhans Cells in the cornea.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

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Langerhans Cells
Interleukin-3
Chemotaxis
Interleukin-4
Cornea
Cytokines
Inbred C3H Mouse
Antigen-Presenting Cells
Interleukin-2
Staining and Labeling
Injections

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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Chemotaxis by various cytokines of Ia+ Langerhans cells into the central cornea of mice : Effect of interleukins 3 and 4. / Epstein, S. P.; Asbell, Penny; Chamie, G.; Davoodzadeh, M.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.

Research output: Contribution to journalArticle

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title = "Chemotaxis by various cytokines of Ia+ Langerhans cells into the central cornea of mice: Effect of interleukins 3 and 4",
abstract = "Purpose: Although the central cornea under normal conditions is devoid of Ia+ Langerhans cells (LC), several cytokines have been shown to induce centripetal migration of LC. We sought to determine whether Interleukins (IL)-3 and IL-4 have an effect upon these antigen presenting cells. Methods: Applications of murine (mu) IL-3 are active in the concentration range of 0.1 to 10.0 ng/ml in vitro and 1.0 to 100.0 ng in vivo. Applications of mu IL-4 are active in the range of 0.5 to 40.0 ng/mL in vitro and 5.0 to 200.0 ng in vivo. BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts (max. 1.0μg) recombinant (r) murine (mu) IL-3 or 250 IU r mu IL-4 and evaluated 16 hours later by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas were found to contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) Ia+ LC peripherally, with none regularly found centrally or paracentrally. Intracorneal injection of r mu IL-3 induced the migration of 0.25±0.6 BALB/c, 0.42±0.5 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC into the cornea; while r mu IL-4 induced the migration of 0.25±0.6 BALB/c, 0.39±0.6 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC. Neither r mu IL-3 (BALB/c: p=0.90; C57BL/6: p=0.90; C3H/HeJ: p=0.92), nor r mu IL-4 (BALB/c: p=0.90; C57BL/6: p-0.99; C3H/HeJ: p=0.92) induced the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied. Conclusions: Prior exploration of the chemotactic cascade has demonstrated cytokine induction of Ia+ Langerhans cells into the central cornea. Unlike other cytokines (ie. IL-2, TNF-α, etc.), we propose that neither IL-3 nor IL-4 are chemotactic for Ia+ Langerhans Cells in the cornea.",
author = "Epstein, {S. P.} and Penny Asbell and G. Chamie and M. Davoodzadeh",
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T1 - Chemotaxis by various cytokines of Ia+ Langerhans cells into the central cornea of mice

T2 - Effect of interleukins 3 and 4

AU - Epstein, S. P.

AU - Asbell, Penny

AU - Chamie, G.

AU - Davoodzadeh, M.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose: Although the central cornea under normal conditions is devoid of Ia+ Langerhans cells (LC), several cytokines have been shown to induce centripetal migration of LC. We sought to determine whether Interleukins (IL)-3 and IL-4 have an effect upon these antigen presenting cells. Methods: Applications of murine (mu) IL-3 are active in the concentration range of 0.1 to 10.0 ng/ml in vitro and 1.0 to 100.0 ng in vivo. Applications of mu IL-4 are active in the range of 0.5 to 40.0 ng/mL in vitro and 5.0 to 200.0 ng in vivo. BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts (max. 1.0μg) recombinant (r) murine (mu) IL-3 or 250 IU r mu IL-4 and evaluated 16 hours later by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas were found to contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) Ia+ LC peripherally, with none regularly found centrally or paracentrally. Intracorneal injection of r mu IL-3 induced the migration of 0.25±0.6 BALB/c, 0.42±0.5 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC into the cornea; while r mu IL-4 induced the migration of 0.25±0.6 BALB/c, 0.39±0.6 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC. Neither r mu IL-3 (BALB/c: p=0.90; C57BL/6: p=0.90; C3H/HeJ: p=0.92), nor r mu IL-4 (BALB/c: p=0.90; C57BL/6: p-0.99; C3H/HeJ: p=0.92) induced the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied. Conclusions: Prior exploration of the chemotactic cascade has demonstrated cytokine induction of Ia+ Langerhans cells into the central cornea. Unlike other cytokines (ie. IL-2, TNF-α, etc.), we propose that neither IL-3 nor IL-4 are chemotactic for Ia+ Langerhans Cells in the cornea.

AB - Purpose: Although the central cornea under normal conditions is devoid of Ia+ Langerhans cells (LC), several cytokines have been shown to induce centripetal migration of LC. We sought to determine whether Interleukins (IL)-3 and IL-4 have an effect upon these antigen presenting cells. Methods: Applications of murine (mu) IL-3 are active in the concentration range of 0.1 to 10.0 ng/ml in vitro and 1.0 to 100.0 ng in vivo. Applications of mu IL-4 are active in the range of 0.5 to 40.0 ng/mL in vitro and 5.0 to 200.0 ng in vivo. BALB/c, C57BL/6 and C3H/HeJ mouse corneas were injected with varying amounts (max. 1.0μg) recombinant (r) murine (mu) IL-3 or 250 IU r mu IL-4 and evaluated 16 hours later by immunoperoxidase staining. Results: Unstimulated 6-8 week old mouse corneas were found to contain 0-3 (BALB/c: 0.28±0.46; C57BL/6: 0.41±0.59; C3H/HeJ: 0.82±0.75) Ia+ LC peripherally, with none regularly found centrally or paracentrally. Intracorneal injection of r mu IL-3 induced the migration of 0.25±0.6 BALB/c, 0.42±0.5 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC into the cornea; while r mu IL-4 induced the migration of 0.25±0.6 BALB/c, 0.39±0.6 C57BL/6 and 0.79±0.8 C3H/HeJ Ia+ LC. Neither r mu IL-3 (BALB/c: p=0.90; C57BL/6: p=0.90; C3H/HeJ: p=0.92), nor r mu IL-4 (BALB/c: p=0.90; C57BL/6: p-0.99; C3H/HeJ: p=0.92) induced the chemotaxis of a statistically significant number of Ia+ LC into the central corneas of the murine strains studied. Conclusions: Prior exploration of the chemotactic cascade has demonstrated cytokine induction of Ia+ Langerhans cells into the central cornea. Unlike other cytokines (ie. IL-2, TNF-α, etc.), we propose that neither IL-3 nor IL-4 are chemotactic for Ia+ Langerhans Cells in the cornea.

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