Chicken Y-box proteins chk-YB-1b and chk-YB-2 repress translation by sequence-specific interaction with single-stranded RNA

Shivalingappa K. Swamynathan, Ashok Nambiar, Ramareddy Guntaka

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Y-Box proteins comprise a large family of multifunctional proteins with a wide spectrum of activities in both transcription and translational regulation of gene expression. Earlier, we have reported on the involvement of chk-YB-2 in transcriptional regulation of Rous sarcoma virus long terminal repeats and the involvement of chk-YB-1b in transcriptional regulation of alpha 1 (I) collagen genes. Here, we have investigated the potential role of chk-YB-2 and chk-YB-1b in RNA metabolism. We report that chk-YB-2 and chk-YB-1b are localized predominantly in the cytoplasm and that they both can bind single-stranded RNA in a sequence-specific and reversible manner. Well-conserved cold-shock domain, N-terminal proline-rich domain and the alternating clusters of acidic and basic amino acids located in the C-terminal ends of these two proteins were all found to be necessary for their RNA-binding ability. Further, we demonstrate that these two proteins inhibit translation in vitro and that binding to RNA is required for this inhibition. The significance of these results is discussed.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalBiochemical Journal
Volume348
Issue number2
DOIs
StatePublished - Jun 1 2000

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Chickens
RNA
Proteins
Acidic Amino Acids
Rous sarcoma virus
Basic Amino Acids
Terminal Repeat Sequences
Gene Expression Regulation
Protein Biosynthesis
Transcription
Viruses
Proline
Metabolism
Gene expression
Shock
Cytoplasm
Collagen
Genes
indenolol

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chicken Y-box proteins chk-YB-1b and chk-YB-2 repress translation by sequence-specific interaction with single-stranded RNA. / Swamynathan, Shivalingappa K.; Nambiar, Ashok; Guntaka, Ramareddy.

In: Biochemical Journal, Vol. 348, No. 2, 01.06.2000, p. 297-305.

Research output: Contribution to journalArticle

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AB - Y-Box proteins comprise a large family of multifunctional proteins with a wide spectrum of activities in both transcription and translational regulation of gene expression. Earlier, we have reported on the involvement of chk-YB-2 in transcriptional regulation of Rous sarcoma virus long terminal repeats and the involvement of chk-YB-1b in transcriptional regulation of alpha 1 (I) collagen genes. Here, we have investigated the potential role of chk-YB-2 and chk-YB-1b in RNA metabolism. We report that chk-YB-2 and chk-YB-1b are localized predominantly in the cytoplasm and that they both can bind single-stranded RNA in a sequence-specific and reversible manner. Well-conserved cold-shock domain, N-terminal proline-rich domain and the alternating clusters of acidic and basic amino acids located in the C-terminal ends of these two proteins were all found to be necessary for their RNA-binding ability. Further, we demonstrate that these two proteins inhibit translation in vitro and that binding to RNA is required for this inhibition. The significance of these results is discussed.

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