Chicken YB-2, a Y-box protein, is a potent activator of Rous sarcoma virus long terminal repeat-driven transcription in avian fibroblasts

S. K. Swamynathan, Ashok Nambiar, Ramareddy Guntaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have previously reported on the cloning and characterization of chk- YB-2, a novel member of the Y-box family of proteins, that binds to the sequence 5'-GTACCACC-3' present on the noncoding strand of the Rous sarcoma virus (RSV) long terminal repeat (LTR) in a single-strand-specific manner. Here, we demonstrate that deletion or mutation of this motif not only eliminates chk-YB-2 binding in vitro but also down-regulates RSV LTR-driven transcription in avian cells. Selective abrogation of chk- YB-2 expression by using antisense oligonucleotides decreased RSV LTR.driven transcription in a promoter-specific manner. This inhibition was not observed when a reporter construct with a deletion in the chk-YB-2 binding site was used. Depletion of cellular chk-YB-2 by transfecting the cells with excess of its recognition sequence oligonucleotides also resulted in reduced transcription from the RSV LTR. Taken together, these results suggest that chk-YB-2 acts as an activator of LTR-promoted transcription in avian cells and that this activation is mediated primarily through the sequence 5'-GTACCACC-3'.

Original languageEnglish (US)
Pages (from-to)2873-2880
Number of pages8
JournalJournal of Virology
Volume71
Issue number4
StatePublished - Apr 1 1997

Fingerprint

Rous sarcoma virus
terminal repeat sequences
Terminal Repeat Sequences
fibroblasts
Chickens
transcription (genetics)
Fibroblasts
chickens
oligonucleotides
Proteins
proteins
cells
Antisense Oligonucleotides
Sequence Deletion
binding sites
molecular cloning
Oligonucleotides
promoter regions
indenolol
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Chicken YB-2, a Y-box protein, is a potent activator of Rous sarcoma virus long terminal repeat-driven transcription in avian fibroblasts. / Swamynathan, S. K.; Nambiar, Ashok; Guntaka, Ramareddy.

In: Journal of Virology, Vol. 71, No. 4, 01.04.1997, p. 2873-2880.

Research output: Contribution to journalArticle

@article{dc0f84ed831b429494f5bd31404927f2,
title = "Chicken YB-2, a Y-box protein, is a potent activator of Rous sarcoma virus long terminal repeat-driven transcription in avian fibroblasts",
abstract = "We have previously reported on the cloning and characterization of chk- YB-2, a novel member of the Y-box family of proteins, that binds to the sequence 5'-GTACCACC-3' present on the noncoding strand of the Rous sarcoma virus (RSV) long terminal repeat (LTR) in a single-strand-specific manner. Here, we demonstrate that deletion or mutation of this motif not only eliminates chk-YB-2 binding in vitro but also down-regulates RSV LTR-driven transcription in avian cells. Selective abrogation of chk- YB-2 expression by using antisense oligonucleotides decreased RSV LTR.driven transcription in a promoter-specific manner. This inhibition was not observed when a reporter construct with a deletion in the chk-YB-2 binding site was used. Depletion of cellular chk-YB-2 by transfecting the cells with excess of its recognition sequence oligonucleotides also resulted in reduced transcription from the RSV LTR. Taken together, these results suggest that chk-YB-2 acts as an activator of LTR-promoted transcription in avian cells and that this activation is mediated primarily through the sequence 5'-GTACCACC-3'.",
author = "Swamynathan, {S. K.} and Ashok Nambiar and Ramareddy Guntaka",
year = "1997",
month = "4",
day = "1",
language = "English (US)",
volume = "71",
pages = "2873--2880",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "4",

}

TY - JOUR

T1 - Chicken YB-2, a Y-box protein, is a potent activator of Rous sarcoma virus long terminal repeat-driven transcription in avian fibroblasts

AU - Swamynathan, S. K.

AU - Nambiar, Ashok

AU - Guntaka, Ramareddy

PY - 1997/4/1

Y1 - 1997/4/1

N2 - We have previously reported on the cloning and characterization of chk- YB-2, a novel member of the Y-box family of proteins, that binds to the sequence 5'-GTACCACC-3' present on the noncoding strand of the Rous sarcoma virus (RSV) long terminal repeat (LTR) in a single-strand-specific manner. Here, we demonstrate that deletion or mutation of this motif not only eliminates chk-YB-2 binding in vitro but also down-regulates RSV LTR-driven transcription in avian cells. Selective abrogation of chk- YB-2 expression by using antisense oligonucleotides decreased RSV LTR.driven transcription in a promoter-specific manner. This inhibition was not observed when a reporter construct with a deletion in the chk-YB-2 binding site was used. Depletion of cellular chk-YB-2 by transfecting the cells with excess of its recognition sequence oligonucleotides also resulted in reduced transcription from the RSV LTR. Taken together, these results suggest that chk-YB-2 acts as an activator of LTR-promoted transcription in avian cells and that this activation is mediated primarily through the sequence 5'-GTACCACC-3'.

AB - We have previously reported on the cloning and characterization of chk- YB-2, a novel member of the Y-box family of proteins, that binds to the sequence 5'-GTACCACC-3' present on the noncoding strand of the Rous sarcoma virus (RSV) long terminal repeat (LTR) in a single-strand-specific manner. Here, we demonstrate that deletion or mutation of this motif not only eliminates chk-YB-2 binding in vitro but also down-regulates RSV LTR-driven transcription in avian cells. Selective abrogation of chk- YB-2 expression by using antisense oligonucleotides decreased RSV LTR.driven transcription in a promoter-specific manner. This inhibition was not observed when a reporter construct with a deletion in the chk-YB-2 binding site was used. Depletion of cellular chk-YB-2 by transfecting the cells with excess of its recognition sequence oligonucleotides also resulted in reduced transcription from the RSV LTR. Taken together, these results suggest that chk-YB-2 acts as an activator of LTR-promoted transcription in avian cells and that this activation is mediated primarily through the sequence 5'-GTACCACC-3'.

UR - http://www.scopus.com/inward/record.url?scp=0030972476&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030972476&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 2873

EP - 2880

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 4

ER -