Childhood brucellosis in Jordan

prevalence and analysis of risk factors

Ahmad M. Al-Majali, Mahmoud Shorman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Objectives: To investigate the seroprevalence of childhood brucellosis in Jordan and to elucidate risk factors associated with seropositivity to Brucella. Methods: Between January 2001 and October 2006, a cross-sectional study of children aged ≤15 years was conducted; a total of 1282 serum samples were collected. Serum samples were analyzed for the presence of antibodies to Brucella antigens using the Rose Bengal plate test (RBPT) and a direct enzyme-linked immunosorbent assay (ELISA). Risk factors associated with seropositivity to Brucella antigens were identified by constructing a multivariate logistic regression model. Results: Of the 1282 serum samples tested, 119 (9.3%) were positive by both RBPT and ELISA tests. When adjusted for the sensitivities and specificities of the two tests, the true seroprevalence was 11.6%. The seroprevalence of Brucella antibodies in Ma'an and Mafraq governorates was significantly higher than in the other governorates. The logistic regression model identified male gender (OR 2.5, 95% CI 1.4, 4.1), age older than 10 years (OR 1.8, 95% CI 1.1, 3.9), living in a village (OR 2.0, 95% CI 1.3, 2.9), and assisting in raising small ruminants (OR 1.6, 95% CI 1.1, 2.6) as risk factors for childhood Brucella seropositivity in Jordan. Conclusion: This study documents the importance of brucellosis in children and further emphasizes the role of small ruminants as an important source for human infection.

Original languageEnglish (US)
Pages (from-to)196-200
Number of pages5
JournalInternational Journal of Infectious Diseases
Volume13
Issue number2
DOIs
StatePublished - Mar 1 2009

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Jordan
Brucella
Brucellosis
Seroepidemiologic Studies
Logistic Models
Rose Bengal
Ruminants
Serum
Enzyme-Linked Immunosorbent Assay
Antigens
Antibodies
Cross-Sectional Studies
Sensitivity and Specificity
Infection

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Childhood brucellosis in Jordan : prevalence and analysis of risk factors. / Al-Majali, Ahmad M.; Shorman, Mahmoud.

In: International Journal of Infectious Diseases, Vol. 13, No. 2, 01.03.2009, p. 196-200.

Research output: Contribution to journalArticle

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abstract = "Objectives: To investigate the seroprevalence of childhood brucellosis in Jordan and to elucidate risk factors associated with seropositivity to Brucella. Methods: Between January 2001 and October 2006, a cross-sectional study of children aged ≤15 years was conducted; a total of 1282 serum samples were collected. Serum samples were analyzed for the presence of antibodies to Brucella antigens using the Rose Bengal plate test (RBPT) and a direct enzyme-linked immunosorbent assay (ELISA). Risk factors associated with seropositivity to Brucella antigens were identified by constructing a multivariate logistic regression model. Results: Of the 1282 serum samples tested, 119 (9.3{\%}) were positive by both RBPT and ELISA tests. When adjusted for the sensitivities and specificities of the two tests, the true seroprevalence was 11.6{\%}. The seroprevalence of Brucella antibodies in Ma'an and Mafraq governorates was significantly higher than in the other governorates. The logistic regression model identified male gender (OR 2.5, 95{\%} CI 1.4, 4.1), age older than 10 years (OR 1.8, 95{\%} CI 1.1, 3.9), living in a village (OR 2.0, 95{\%} CI 1.3, 2.9), and assisting in raising small ruminants (OR 1.6, 95{\%} CI 1.1, 2.6) as risk factors for childhood Brucella seropositivity in Jordan. Conclusion: This study documents the importance of brucellosis in children and further emphasizes the role of small ruminants as an important source for human infection.",
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