Chitin enhances obese inflammation ex vivo

Chun Jung Huang, Kathleen N. Beasley, Edmund O. Acevedo, Robert L. Franco, Tamekia Jones, David C. Mari, Yoshimi Shibata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Infection has been implicated as a co-risk factor for obesity, but the mechanism remains uncertain. Elevated levels of plasma chitinase 3-like 1 (CHI3L1) are found in obese individuals. Since CHI3L1 is produced by activated immune cells including macrophages and recognizes microbial N-acetylglucosamine polymer (chitin), we asked whether the plasma CHI3L1 protein change in obese individuals might alter their innate immune response to chitin. Thirty-six subjects (15 obese and 21 non-obese), ages 18-30. years, were recruited. Peripheral blood mononuclear cells (PBMCs) were cultured with chitin microparticles (CMP; 1-10. μm) for 24. h; tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), and CHI3L1 in the culture supernatants were measured. We chose CMP, since neither large chitin beads (40-100. μm), chitosan microparticles (1-10. μm), nor soluble chitin induced the cytokine/CHI3L1 production by PBMCs isolated from non-obese PBMCs ex vivo. We found that the quantity of IL-6, but not TNF-α or CHI3L1, induced by CMP was significantly correlated with plasma IL-6, BMI, waist/hip circumferences, fasting plasma insulin, and insulin resistance. These findings suggest that chitin, a substrate of CHI3L1, further promotes obese inflammation in a size- and chemical composition- dependent manner.

Original languageEnglish (US)
Pages (from-to)41-46
Number of pages6
JournalHuman Immunology
Volume75
Issue number1
DOIs
StatePublished - Jan 1 2014

Fingerprint

Chitinases
Chitin
Inflammation
Cytidine Monophosphate
Interleukin-6
Blood Cells
Tumor Necrosis Factor-alpha
Acetylglucosamine
Chitosan
Waist Circumference
Innate Immunity
Insulin Resistance
Hip
Fasting
Polymers
Obesity
Macrophages
Insulin
Cytokines
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Huang, C. J., Beasley, K. N., Acevedo, E. O., Franco, R. L., Jones, T., Mari, D. C., & Shibata, Y. (2014). Chitin enhances obese inflammation ex vivo. Human Immunology, 75(1), 41-46. https://doi.org/10.1016/j.humimm.2013.09.005

Chitin enhances obese inflammation ex vivo. / Huang, Chun Jung; Beasley, Kathleen N.; Acevedo, Edmund O.; Franco, Robert L.; Jones, Tamekia; Mari, David C.; Shibata, Yoshimi.

In: Human Immunology, Vol. 75, No. 1, 01.01.2014, p. 41-46.

Research output: Contribution to journalArticle

Huang, CJ, Beasley, KN, Acevedo, EO, Franco, RL, Jones, T, Mari, DC & Shibata, Y 2014, 'Chitin enhances obese inflammation ex vivo', Human Immunology, vol. 75, no. 1, pp. 41-46. https://doi.org/10.1016/j.humimm.2013.09.005
Huang CJ, Beasley KN, Acevedo EO, Franco RL, Jones T, Mari DC et al. Chitin enhances obese inflammation ex vivo. Human Immunology. 2014 Jan 1;75(1):41-46. https://doi.org/10.1016/j.humimm.2013.09.005
Huang, Chun Jung ; Beasley, Kathleen N. ; Acevedo, Edmund O. ; Franco, Robert L. ; Jones, Tamekia ; Mari, David C. ; Shibata, Yoshimi. / Chitin enhances obese inflammation ex vivo. In: Human Immunology. 2014 ; Vol. 75, No. 1. pp. 41-46.
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