Chk-YB-1b, a Y-box binding protein activates transcription from rat α1(I) procollagen gene promoter

Arvinder K. Dhalla, Seth S. Ririe, Shivalingappa K. Swamynathan, Karl Weber, Ramareddy Guntaka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Type-I collagen, the predominant component of extracellular matrix, is a triple-helical protein consisting of two α1 polypeptides and one α2 polypeptide. Expression of α1 and α2 procollagen genes is co-ordinately regulated under both normal and various pathological conditions. However, the basis of this co-ordinate regulation is not well known. YB-1b, a Y-box protein, has been shown to bind to the polypyrimidine tract present in the α2 procollagen gene. Here, we show that chk-YB-1b, a YB-1 homologue, binds in a single-strand-sequence-specific manner to the highly conserved pyrimidine-rich sequences in both α1(I) and α2(I) procollagen promoters from different species, as demonstrated by electrophoretic-mobility-shift assays and by DNaseI footprinting experiments. Transiently transfected and retrovirally expressed antisense oligonucleotides directed against chk-YB-1b specifically inhibited the α1(I) procollagen promoter-driven transcription in cultured fibroblasts. Considering these data and the fact that the chk-YB-1b binding site is one of the few sites between α1(I) and α2(I) procollagen promoters that is conserved from chicken to human, it is proposed that chk-YB-1b may be involved in co-ordinate expression of these two collagen genes.

Original languageEnglish (US)
Pages (from-to)373-379
Number of pages7
JournalBiochemical Journal
Volume336
Issue number2
DOIs
StatePublished - Dec 1 1998

Fingerprint

Procollagen
Transcription
Rats
Carrier Proteins
Genes
Electrophoretic mobility
Peptides
Antisense Oligonucleotides
Electrophoretic Mobility Shift Assay
Fibroblasts
Collagen Type I
Extracellular Matrix
Chickens
Assays
Proteins
Collagen
Binding Sites
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Chk-YB-1b, a Y-box binding protein activates transcription from rat α1(I) procollagen gene promoter. / Dhalla, Arvinder K.; Ririe, Seth S.; Swamynathan, Shivalingappa K.; Weber, Karl; Guntaka, Ramareddy.

In: Biochemical Journal, Vol. 336, No. 2, 01.12.1998, p. 373-379.

Research output: Contribution to journalArticle

Dhalla, Arvinder K. ; Ririe, Seth S. ; Swamynathan, Shivalingappa K. ; Weber, Karl ; Guntaka, Ramareddy. / Chk-YB-1b, a Y-box binding protein activates transcription from rat α1(I) procollagen gene promoter. In: Biochemical Journal. 1998 ; Vol. 336, No. 2. pp. 373-379.
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