Cholecystokinin antagonists inhibit in vivo voltammetric signals generated by KCl-induced slow wave depolarization in rat caudate

James W. Wilkerson, Richard Kasser, Kenneth J. Renner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of sulfated cholecystokinin octapeptide (CCK-8S) on the generation of slow wave depolarisation in the rat caudate-putamen (CPu) was studied using in vivo voltammetry. Pressure-ejection of 50 μM CCK-8S into the CPu induced voltammetric signals recorded at widely spaced Nafion-coated carbon fiber microelectrodes. Based on the in vitro selectivity properties of the electrodes, the signals were predominantly due to increases in extracellular concentrations of dopamine (DA). The similar propagation rates of the signals induced by CCK-8S and 100 mM KCl suggests that the CCK-8S-induced signals represent a slow wave depolarization (SWD). Since the CPu was refractory to a second CCK-8S stimulus, the effects of CCK antagonists on DA signals associated with 100 mM KCl-induced SWD were evaluated. Proglumide (4-64 mg/kg) and lorglumide (20-640 μg/kg), administered intravenously, decreased KCl-induced DA signals in the CPu in a dose-dependent manner. The antagonistic effect of lorglumide on the KCl-induced signals was partly reversed 130 min after drug administration. The generation of a SWD by CCK-8S and the inhibitory effects of CCK-8S antagonists on KCl-induced signals suggest that the susceptibility of the CPu to KCl-induced SWD may be enhanced by CCK-8S.

Original languageEnglish (US)
Pages (from-to)47-55
Number of pages9
JournalBrain Research
Volume594
Issue number1
DOIs
StatePublished - Oct 23 1992

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Cholecystokinin
Putamen
Dopamine
Proglumide
Sincalide
Dopamine Antagonists
8-sulfocholecystokinin octapeptide
Microelectrodes
Electrodes
Pressure
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Cholecystokinin antagonists inhibit in vivo voltammetric signals generated by KCl-induced slow wave depolarization in rat caudate. / Wilkerson, James W.; Kasser, Richard; Renner, Kenneth J.

In: Brain Research, Vol. 594, No. 1, 23.10.1992, p. 47-55.

Research output: Contribution to journalArticle

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