Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans

Laura A. Woollett, Donna D. Buckley, Lihang Yao, Peter J.H. Jones, Norman A. Granholm, Elizabeth Tolley, Patrick Tso, James E. Heubi

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background & Aims: Qualitative and quantitative changes in Intralumenal bile acid composition may alter cholesterol absorption and synthesis and low-density lipoprotein (LDL) receptor expression. The role of cholic acid (CA) in cholesterol absorption in humans remains unclear and, thus, was examined in the current study. Methods: In a crossover design outpatient study, 12 adults aged 24-36 years took 15 mg/kg/day (CA) or no bile acid supplement (control) while being fed a controlled diet (AHA heart-healthy diet). A liquid meal of defined composition was given on day 14 of the diet, and lumenal samples were collected. Thereafter, cholesterol absorption and cholesterol fractional synthetic rate (FSR) were assessed by stable isotopic methods from days 16 to 20. Results: With CA treatment, bile was enriched significantly with CA (P < 0.0004) to 60.2% ± 2.4% (mean ± SEM) compared with 43.3% ± 2.4% for controls. CA plus diet treatment significantly increased (P = 0.013) cholesterol absorption (72.6% ± 2.9%) compared with diet treatment alone (60.4% ± 2.9%). Percentage micellar cholesterol was increased by CA plus diet treatment vs. diet alone after meal ingestion (P = 0.004). Plasma total and high-density lipoprotein (HDL) and LDL cholesterol was unchanged with CA treatment. Conclusions: Thus, enrichment in lumenal bile with CA results in an increase in cholesterol absorption, an effect potentially mediated by enhanced cholesterol solubilization in micelles.

Original languageEnglish (US)
Pages (from-to)724-731
Number of pages8
JournalGastroenterology
Volume126
Issue number3
DOIs
StatePublished - Jan 1 2004

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Cholic Acid
Cholesterol
Diet
Bile Acids and Salts
Bile
Meals
Therapeutics
LDL Receptors
Micelles
HDL Lipoproteins
LDL Cholesterol
Cross-Over Studies
Outpatients
Eating

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Woollett, L. A., Buckley, D. D., Yao, L., Jones, P. J. H., Granholm, N. A., Tolley, E., ... Heubi, J. E. (2004). Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans. Gastroenterology, 126(3), 724-731. https://doi.org/10.1053/j.gastro.2003.11.058

Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans. / Woollett, Laura A.; Buckley, Donna D.; Yao, Lihang; Jones, Peter J.H.; Granholm, Norman A.; Tolley, Elizabeth; Tso, Patrick; Heubi, James E.

In: Gastroenterology, Vol. 126, No. 3, 01.01.2004, p. 724-731.

Research output: Contribution to journalArticle

Woollett, LA, Buckley, DD, Yao, L, Jones, PJH, Granholm, NA, Tolley, E, Tso, P & Heubi, JE 2004, 'Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans', Gastroenterology, vol. 126, no. 3, pp. 724-731. https://doi.org/10.1053/j.gastro.2003.11.058
Woollett LA, Buckley DD, Yao L, Jones PJH, Granholm NA, Tolley E et al. Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans. Gastroenterology. 2004 Jan 1;126(3):724-731. https://doi.org/10.1053/j.gastro.2003.11.058
Woollett, Laura A. ; Buckley, Donna D. ; Yao, Lihang ; Jones, Peter J.H. ; Granholm, Norman A. ; Tolley, Elizabeth ; Tso, Patrick ; Heubi, James E. / Cholic Acid Supplementation Enhances Cholesterol Absorption in Humans. In: Gastroenterology. 2004 ; Vol. 126, No. 3. pp. 724-731.
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