Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models

Danielle L. Brooks, Tiffany Seagroves

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful method to determine whether a protein of interest binds to specific regulatory elements of the genome. Herein, we outline protocols optimized to detect binding of Hypoxia-Inducible Factor (HIF)-1α or HIF-2α to putative hypoxia response elements (HREs) within HIF target genes expressed in breast tumor epithelial cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages67-79
Number of pages13
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1742
ISSN (Print)1064-3745

Fingerprint

Chromatin Immunoprecipitation
Breast Neoplasms
Hypoxia-Inducible Factor 1
Response Elements
Epithelial Cells
Genome
Genes
Proteins
Hypoxia
endothelial PAS domain-containing protein 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Brooks, D. L., & Seagroves, T. (2018). Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. In Methods in Molecular Biology (pp. 67-79). (Methods in Molecular Biology; Vol. 1742). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7665-2_7

Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. / Brooks, Danielle L.; Seagroves, Tiffany.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 67-79 (Methods in Molecular Biology; Vol. 1742).

Research output: Chapter in Book/Report/Conference proceedingChapter

Brooks, DL & Seagroves, T 2018, Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1742, Humana Press Inc., pp. 67-79. https://doi.org/10.1007/978-1-4939-7665-2_7
Brooks DL, Seagroves T. Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 67-79. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7665-2_7
Brooks, Danielle L. ; Seagroves, Tiffany. / Chromatin immunoprecipitation of HIF-α in breast tumor cells using wild type and loss of function models. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 67-79 (Methods in Molecular Biology).
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