CIP2A and PP2A in human leptomeninges, arachnoid granulations and meningiomas

Mahlon Johnson, Jay E. Reeder, Mary O'Connell, Mark Woodford, Kevin Walter

Research output: Contribution to journalArticle

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Abstract

Previously we have found that mitogens stimulate proliferation of meningioma cells of all grades, in part, by activation of the PI3K-PKB/Akt-PRAS40-mTOR pathway regulated to some degree by the tumor suppressor phosphatase PP2A. PP2A activity is inhibited by the oncoprotein cancerous inhibitor of PP2A (CIP2A), which has not been studied in meningiomas to our knowledge. Six fetal and one adult human leptomeningeal samples and 38 meningiomas were evaluated by western blot. Fifteen adult arachnoid granulations and 58 formalin-fixed meningiomas (36 World Health Organization grade I, 15 grade II and seven grade III) were evaluated by immunohistochemistry. The effects of the mitogens platelet derived growth factor-BB (PDGF-BB) and cerebrospinal fluid on CIP2A were also studied. By western blot, CIP2A and PP2A were found in the five fetal and one adult leptomeninges and all meningiomas. By immunohistochemistry, CIP2A was detected in the arachnoid granulations and all meningiomas. CIP2A tended to be higher in grade III tumors. Three fetal leptomeningeal (two grade I and one grade II) and meningioma cells treated with PDGF-BB and/or human cerebrospinal fluid resulted in a slight increase in CIP2A in the leptomeningeal cells but not meningioma cells. Considered the mechanism of action and seen in other neoplasms, these findings raise the possibility that CIP2A may participate in the biology of meningiomas.

Original languageEnglish (US)
Pages (from-to)2228-2232
Number of pages5
JournalJournal of Clinical Neuroscience
Volume21
Issue number12
DOIs
StatePublished - Dec 1 2014

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Arachnoid
Meningioma
Mitogens
Cerebrospinal Fluid
Western Blotting
Immunohistochemistry
Neoplasms
Oncogene Proteins
Phosphatidylinositol 3-Kinases
Phosphoric Monoester Hydrolases
Formaldehyde
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Surgery
  • Neurology
  • Clinical Neurology
  • Physiology (medical)

Cite this

CIP2A and PP2A in human leptomeninges, arachnoid granulations and meningiomas. / Johnson, Mahlon; Reeder, Jay E.; O'Connell, Mary; Woodford, Mark; Walter, Kevin.

In: Journal of Clinical Neuroscience, Vol. 21, No. 12, 01.12.2014, p. 2228-2232.

Research output: Contribution to journalArticle

Johnson, Mahlon ; Reeder, Jay E. ; O'Connell, Mary ; Woodford, Mark ; Walter, Kevin. / CIP2A and PP2A in human leptomeninges, arachnoid granulations and meningiomas. In: Journal of Clinical Neuroscience. 2014 ; Vol. 21, No. 12. pp. 2228-2232.
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