Class I antibody induction by rat endothelium

Mitchell Goldman, N. Tuttle-Fuller, Josephine Salim, Stephen Bendheim, Thalachallour Mohanakumar

Research output: Contribution to journalArticle

Abstract

Supradiaphragmatic vein grafts were transplanted from ACI (RT1a) to Fisher (RT11) rats to determine whether the endothelium and/or circulating passenger leukocytes were necessary for the induction of an antibody response. Vein grafts from immunosuppressed and irradiated donors induced lymphocytotoxic and hemagglutinating antibody in recipient rats. In addition, IgG antibody produced in Fisher recipients was found by indirect immunofluorescence to donor endothelium. When the endothelium was removed from donor veins either by collagenase or by scraping the intima, no antibody resulted after transplantation. The lymphocytotoxic and hemagglutinating antibodies and the antibodies identified by indirect immunofluorescence were removed by absorbing the Fisher serum in ACI red blood cells. The endothelium seems to be capable of inducing Class I antibodies when allogeneically transplanted as vein grafts.

Original languageEnglish (US)
Pages (from-to)327-331
Number of pages5
JournalJournal of Surgical Research
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Immunoglobulin Isotypes
Endothelium
Veins
Antilymphocyte Serum
Indirect Fluorescent Antibody Technique
Transplants
Antibodies
Collagenases
Antibody Formation
Leukocytes
Immunoglobulin G
Transplantation
Erythrocytes
Serum

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Goldman, M., Tuttle-Fuller, N., Salim, J., Bendheim, S., & Mohanakumar, T. (1984). Class I antibody induction by rat endothelium. Journal of Surgical Research, 36(4), 327-331. https://doi.org/10.1016/0022-4804(84)90107-0

Class I antibody induction by rat endothelium. / Goldman, Mitchell; Tuttle-Fuller, N.; Salim, Josephine; Bendheim, Stephen; Mohanakumar, Thalachallour.

In: Journal of Surgical Research, Vol. 36, No. 4, 01.01.1984, p. 327-331.

Research output: Contribution to journalArticle

Goldman, M, Tuttle-Fuller, N, Salim, J, Bendheim, S & Mohanakumar, T 1984, 'Class I antibody induction by rat endothelium', Journal of Surgical Research, vol. 36, no. 4, pp. 327-331. https://doi.org/10.1016/0022-4804(84)90107-0
Goldman M, Tuttle-Fuller N, Salim J, Bendheim S, Mohanakumar T. Class I antibody induction by rat endothelium. Journal of Surgical Research. 1984 Jan 1;36(4):327-331. https://doi.org/10.1016/0022-4804(84)90107-0
Goldman, Mitchell ; Tuttle-Fuller, N. ; Salim, Josephine ; Bendheim, Stephen ; Mohanakumar, Thalachallour. / Class I antibody induction by rat endothelium. In: Journal of Surgical Research. 1984 ; Vol. 36, No. 4. pp. 327-331.
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